MOLECULAR CHARACTERIZATION AND O- SEROTYPING IN VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTION IN TEHRAN

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HOSSEINI FARZANEH; MOHAJERI AMIRI M.; BAMBAEI B.; MORADI BIDHENDI S.

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2010 | Volume:32 | Issue:1 Page(s): 39-44

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Title

MOLECULAR CHARACTERIZATION AND O- SEROTYPING IN VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTION IN TEHRAN

Author(s)

HOSSEINI FARZANEH | MOHAJERI AMIRI M. | BAMBAEI B. | MORADI BIDHENDI S. | Issue Writer Certificate

Keywords

ESCHERICHIA COLIQ2

VEROTOXINQ3

PCRQ2

SEROTYPINGQ3

Abstract

Background and Objectives: Verocytotoxin-producing: ESCHERICHIA COLI strains are important human pathogens. Here we studied the prevalence of virulence genes and O stereotyping in strains isolated from urine samples.Materials and Methods: In this study, 300 urine samples from individuals with urinary tract infections E. coli was isolated using enterobacteriase diagnostic kit. The strains were examined with polyvalent and monovalant antiserum for O-serotyping. Verocytotoxin-producing E. coliv strains were characterized by polymerase chain reaction. O157 groups were distinguished from non O157 groups by culturing in sorbitol macconkey agar. Rapid Passive Latex Agglutination (RPLA) technique was performed in order to find the expression of vtx1 and vtx2 genes. Results: From total number of 300 urine samples, 180 samples had positive culture result for E. coli of these, 158 strains had virulence genes and all of them showed a toxic effect after applying Rapid Passive Latex Agglutination technique. Twenty four percent of samples were classified as O157 group and 76% as non O157 group. The most prevalent virulence genes were found in O26 strains containing vtx1 genes. Conclusion: Our findings showed that non-O157 verocytotoxin-producing E.Coli is more prevalent than O157 strains in our study.

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APA: Copy

HOSSEINI, FARZANEH, MOHAJERI AMIRI, M., BAMBAEI, B., & MORADI BIDHENDI, S.. (2010). MOLECULAR CHARACTERIZATION AND O- SEROTYPING IN VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTION IN TEHRAN. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 32(1), 39-44. SID. https://sid.ir/paper/46460/en

Vancouver: Copy

HOSSEINI FARZANEH, MOHAJERI AMIRI M., BAMBAEI B., MORADI BIDHENDI S.. MOLECULAR CHARACTERIZATION AND O- SEROTYPING IN VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTION IN TEHRAN. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2010;32(1):39-44. Available from: https://sid.ir/paper/46460/en

IEEE: Copy

FARZANEH HOSSEINI, M. MOHAJERI AMIRI, B. BAMBAEI, and S. MORADI BIDHENDI, “MOLECULAR CHARACTERIZATION AND O- SEROTYPING IN VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTION IN TEHRAN,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 32, no. 1, pp. 39–44, 2010, [Online]. Available: https://sid.ir/paper/46460/en

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