EFFECT OF VITAMINS C, E AND B-CAROTENE ON IRON-INDUCED DNA DAMAGE IN CACO-2 CELLS

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POURGHASEM GARGARI B.; BEHZAD M.H.; SAFAIYAN A.; ANDERSON D.

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Journal: Medical Journal of Tabriz University of Medical Sciences Year:2006 | Volume:28 | Issue:2 Page(s): 31-37

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Title

EFFECT OF VITAMINS C, E AND B-CAROTENE ON IRON-INDUCED DNA DAMAGE IN CACO-2 CELLS

Author(s)

POURGHASEM GARGARI B. | BEHZAD M.H. | SAFAIYAN A. | ANDERSON D. | Issue Writer Certificate

Keywords

COMMET ASSAYQ3

DNA DAMAGEQ3

IRONQ3

VITAMINS CQ3

EQ3

B-CAROTENEQ3

Abstract

Background and objectives: Role and interaction of IRON and antioxidant vitamins on DNA DAMAGE are not clear, so we decided to determine the Effect of IRON only (as Fe+3) or together with Nitrilotriacetic acid (NTA) on DNA, and then assay Effects of vitamin C, E and b - carotene on IRON induced DNA DAMAGE in an In vitro model.Materials and Methods: Caco-2 cells, the model of gut Epithelium, were incubated with medium containing supplemented IRON (0-560 micromole/Litter) as FeCl3 or IRON plus NTA (Fe/NTA) for 30 minutes, and then DNA DAMAGE was measured. In the second part of study, the cells were incubated with 160 mM/L of IRON (as Fe+3/ NTA) together with various doses of vitamin C (0-320 m M/L), E(0-320 mM/L), or B-CAROTENE (0-12 mM/L).Then DNA DAMAGE was measured.Results: Our study showed IRON as Fe3+ doesn't have any Effect on DNA, while together with NTA, caused significant DNA DAMAGE at doses higher than 160 mM/L. VITAMINS C and E at low doses (2.5 and 2.5-10 m M/L, respectively), decreased IRON-induced DNA DAMAGE(p<0.05). b- carotene doesn't have significant Effect in reducing of IRON-induced DNA DAMAGE. At higher doses, all vitamins increased IRON-induced DNA DAMAGE (p<0.05).Conclusion: In this in vitro model, soluble IRON at high concentrations can cause DNA DAMAGE. In the presence of high concentration of antioxidant vitamins, this Effect of IRON can be strengthened.

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APA: Copy

POURGHASEM GARGARI, B., BEHZAD, M.H., SAFAIYAN, A., & ANDERSON, D.. (2006). EFFECT OF VITAMINS C, E AND B-CAROTENE ON IRON-INDUCED DNA DAMAGE IN CACO-2 CELLS. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 28(2), 31-37. SID. https://sid.ir/paper/47221/en

Vancouver: Copy

POURGHASEM GARGARI B., BEHZAD M.H., SAFAIYAN A., ANDERSON D.. EFFECT OF VITAMINS C, E AND B-CAROTENE ON IRON-INDUCED DNA DAMAGE IN CACO-2 CELLS. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2006;28(2):31-37. Available from: https://sid.ir/paper/47221/en

IEEE: Copy

B. POURGHASEM GARGARI, M.H. BEHZAD, A. SAFAIYAN, and D. ANDERSON, “EFFECT OF VITAMINS C, E AND B-CAROTENE ON IRON-INDUCED DNA DAMAGE IN CACO-2 CELLS,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 2, pp. 31–37, 2006, [Online]. Available: https://sid.ir/paper/47221/en

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