EXTRACTION AND PURIFICATION OF DNA POLYMERASE ENZYME FROM BACILLUS STEAROTHERMOPHILUS ISOLATED FROM GAVMISH GOLY HOT SPRING IN SARAEYN CITY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ASGHARZADEH M.; HOUSSEIN POUR FAIZI M.A.; RAFI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: PHARMACEUTICAL SCIENCES Year:2004 | Volume:- | Issue:2 Page(s): 61-68

Download Full-Text

Persian Verion

View:

2,403

Download:

Cites:

Information Journal Paper

Title

EXTRACTION AND PURIFICATION OF DNA POLYMERASE ENZYME FROM BACILLUS STEAROTHERMOPHILUS ISOLATED FROM GAVMISH GOLY HOT SPRING IN SARAEYN CITY

Author(s)

ASGHARZADEH M. | HOUSSEIN POUR FAIZI M.A. | RAFI A. | Issue Writer Certificate

Keywords

BACILLUS STEAROTHERMOPHLIUSQ3

DNA POLYMERASE ENZYMEQ3

PURIFICATIONQ3

Abstract

DNA Polymerase enzyme undertake the DNA replication and in molecular research DNA POLYMERASE ENZYMEs especially thermostable enzymes have great importance. In this research, thermostable DNA polymerase was extracted and purified from Bacillus stearothermophilus isolated from Gavmish Goly hot spring in Saraeyn city. Bacteria were cultured in Luria-Bertani broth at 55°C and harvested in logarithm phase. Twenty grams of cell paste obtained and resuspended in related buffer. Crude cell extracts were prepared by using of lysozyme and sonication. PURIFICATION were performed by ammonium sulfate precipitation and three steps of chromatography with DEAE-sephadex, phosphocellulose and Heparin-sepharose. DNA POLYMERASE ENZYME was purified 127-fold and specific activity of the purified enzyme was 223 (U/mg). DNA polymerase had the most activity at 55°C. Three protein bands were obtained by using of SDS-P AGE. The major band had a molecular weight of approximately 76000 daltons. BACILLUS STEAROTHERMOPHLIUS DNA polymerase can use in DNA replication, site directed mutagenesis and probably in DNA sequencing at high temperature.

Cites

DESULFOTOMACULUM, AN ANAEROBIC THERMOPHILIC SULFATE-REDUCING BACTERIUM ISOLATED FROM SARISU MINERAL HOT SPRING IN ARDEBIL PROVINCE

References

No record.

Cite

APA: Copy

ASGHARZADEH, M., HOUSSEIN POUR FAIZI, M.A., & RAFI, A.. (2004). EXTRACTION AND PURIFICATION OF DNA POLYMERASE ENZYME FROM BACILLUS STEAROTHERMOPHILUS ISOLATED FROM GAVMISH GOLY HOT SPRING IN SARAEYN CITY. PHARMACEUTICAL SCIENCES, -(2), 61-68. SID. https://sid.ir/paper/48482/en

Vancouver: Copy

ASGHARZADEH M., HOUSSEIN POUR FAIZI M.A., RAFI A.. EXTRACTION AND PURIFICATION OF DNA POLYMERASE ENZYME FROM BACILLUS STEAROTHERMOPHILUS ISOLATED FROM GAVMISH GOLY HOT SPRING IN SARAEYN CITY. PHARMACEUTICAL SCIENCES[Internet]. 2004;-(2):61-68. Available from: https://sid.ir/paper/48482/en

IEEE: Copy

M. ASGHARZADEH, M.A. HOUSSEIN POUR FAIZI, and A. RAFI, “EXTRACTION AND PURIFICATION OF DNA POLYMERASE ENZYME FROM BACILLUS STEAROTHERMOPHILUS ISOLATED FROM GAVMISH GOLY HOT SPRING IN SARAEYN CITY,” PHARMACEUTICAL SCIENCES, vol. -, no. 2, pp. 61–68, 2004, [Online]. Available: https://sid.ir/paper/48482/en

Related Journal Papers