MEASUREMENT OF OXIDIZED GLUTATHIONE LEVEL IN RAT PLASMA EXPOSED TO PARAQUAT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAMI Z.; AMINI MOHSEN; KIANI A.; GHAZI KHANSARI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Isfahan Medical School Year:2008 | Volume:25 | Issue:87 Page(s): 36-44

Download Full-Text

Persian Verion

View:

838

Download:

Cites:

Information Journal Paper

Title

MEASUREMENT OF OXIDIZED GLUTATHIONE LEVEL IN RAT PLASMA EXPOSED TO PARAQUAT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD

Author(s)

HAMI Z. | AMINI MOHSEN | KIANI A. | GHAZI KHANSARI M. | Issue Writer Certificate

Keywords

OXIDIZED GLUTATHIONE (GSSG)Q3

PARAQUATQ2

9-FLUOTENYLMETHYLQ3

CHLOROFORMATE (FMOC)Q3

HPLCQ3

Abstract

Background: PARAQUAT which is used as herbicide, decreases reduced glutathione (GSH) and increases oxidized glutathione (GSSG) via oxidative stress pathway and formation of ROS (Reactive Oxygen Species). High performance liquid chromatography (HPLC) with fluorescence detector is one of the best methods for assessment of glutathione in samples like plasma which contains low level of this substance for evaluation of many of diseases. The purpose of this study was to evaluate the changes of glutathione quantity due to effect of PARAQUAT in rat plasma with the use of HPLC.Methods: In this study the fluorescence substance 9-fluorenylmethylchloroformate (FMOC) was used for derivatization of amine group of glutathione. To do this, the rat obtained plasma, which had been exposed with four doses of PARAQUAT (20, 30, 40, and 60 mg/kg), was mixed with acetonitryl and centrifuged at 5000 rpm for protein precipitation. The supernatant was completely dehydrated in a 40°C water bath under air flow with an air pump. 50ml tap water, 50ml borate buffer 0.5M, pH=9 and 100 ml FMOC 500 mg/ml were added and then was left at room temperature for 15 min for completion of derivatization. After that, 20ml of this solution was injected into a HPLC with C18 column, mobile phase methanol: phosphate buffer (60:40 v/v) and lex=260nm, lem=315nm.Findings: According to the obtained results, the amount of oxidized glutathione was increased significantly only in the dose of 60 mg/kg in comparison with the control group (p<0.05).Conclusion: This method of derivatization and determination of glutathione was successful but to reach to more quantitative and more qualitative results, more studies in the field of determination and selection of chromatography situations are necessary.

Cites

No record.

References

No record.

Cite

APA: Copy

HAMI, Z., AMINI, MOHSEN, KIANI, A., & GHAZI KHANSARI, M.. (2008). MEASUREMENT OF OXIDIZED GLUTATHIONE LEVEL IN RAT PLASMA EXPOSED TO PARAQUAT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 25(87), 36-44. SID. https://sid.ir/paper/50473/en

Vancouver: Copy

HAMI Z., AMINI MOHSEN, KIANI A., GHAZI KHANSARI M.. MEASUREMENT OF OXIDIZED GLUTATHIONE LEVEL IN RAT PLASMA EXPOSED TO PARAQUAT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2008;25(87):36-44. Available from: https://sid.ir/paper/50473/en

IEEE: Copy

Z. HAMI, MOHSEN AMINI, A. KIANI, and M. GHAZI KHANSARI, “MEASUREMENT OF OXIDIZED GLUTATHIONE LEVEL IN RAT PLASMA EXPOSED TO PARAQUAT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 25, no. 87, pp. 36–44, 2008, [Online]. Available: https://sid.ir/paper/50473/en

Related Journal Papers