THE EFFECT OF PH AND TEMPERATURE ON AXENIC CULTURE MEDIA OF LEISHMANIA AMASTIGOTES

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HEJAZI S.H.A.D.; SOLEYMANIFARD S.; CHABAVIZADEH J.

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2004 | Volume:22 | Issue:72 Page(s): 15-19

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Title

THE EFFECT OF PH AND TEMPERATURE ON AXENIC CULTURE MEDIA OF LEISHMANIA AMASTIGOTES

Author(s)

HEJAZI S.H.A.D. | SOLEYMANIFARD S. | CHABAVIZADEH J. | Issue Writer Certificate

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Abstract

Introduction. Leishmaniasisi is a group of diseases that caused by protozoa of the genus- Leishmania related to trypanosomatidae family. Promastigote forms or leptomunas of the parasite is transfered to vertebrate host including human by the bite of infectious sandfly and sheltered in reticuloendothelial cells which multiplies and changes to immobile amastigote form. It is feasible to culture and mass production of promastigotes in semi solid, biphasic and liquid media. For the reason that there are many differences between promastigote form and amastigote form in metabolical, Biochemical and enzymatical characters. Data obtained from studies on amastigotes which are the main of the parasite in human body can resulted in detail data about the parasite. Feasible access to this from of parasite can help very much for successful control of Leishmaniasis, especially designing ways for therapeutic methods.Method. L. major (MRHOIIR/75/ER) from previously infected Balb/c mice was transfered to modified N.N.N medium with overlay of liquid RH.LR After isolation and growth at 26±1°C promastigote from of parasites were transfered to liquid cell culture medium, RPMI 1640. Culture media with PH: 3.5, 4.5, 5.5 (±0.1) were provided and 5xl06 parasites were added to each medium. All cultures were incubated for 24 in 25 and then were 28°c, 32°c and 37°c. The above procedure was repeated with needed changes many times unitl finally amastigote forms were resulted from fellagelated forms of the parasite.Results. The most suitable media was media with PH: 3.5 and most suitable temperature was 37°c which caused the most number of amastigote in vitro.Discussion. There are other methods that can obtain amastigotes in vitro. For example culture of parasite in a media containing macrophages from peritoneal cavity of hamsters or other cell sources lik human and cells. But this methods have draw back as extratime consuming, high price lab equipments, high expenses and contamination by host materials, so that the benefit of peresent study to the mentioned methods is really cleared.

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APA: Copy

HEJAZI, S.H.A.D., SOLEYMANIFARD, S., & CHABAVIZADEH, J.. (2004). THE EFFECT OF PH AND TEMPERATURE ON AXENIC CULTURE MEDIA OF LEISHMANIA AMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 22(72), 15-19. SID. https://sid.ir/paper/50692/en

Vancouver: Copy

HEJAZI S.H.A.D., SOLEYMANIFARD S., CHABAVIZADEH J.. THE EFFECT OF PH AND TEMPERATURE ON AXENIC CULTURE MEDIA OF LEISHMANIA AMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2004;22(72):15-19. Available from: https://sid.ir/paper/50692/en

IEEE: Copy

S.H.A.D. HEJAZI, S. SOLEYMANIFARD, and J. CHABAVIZADEH, “THE EFFECT OF PH AND TEMPERATURE ON AXENIC CULTURE MEDIA OF LEISHMANIA AMASTIGOTES,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 22, no. 72, pp. 15–19, 2004, [Online]. Available: https://sid.ir/paper/50692/en

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