APOPTOTIC EFFECT OF TAMOXIFEN IN THE INFILTRATING BREAST ADENOCARCINOMA MDA-MB468 CELLS: THE INVOLVEMENT OF CASPASE-3, BCL-2 AND BAX PROTEINS

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KARAMI TEHRANI FATEMEH; SALAMI S.; OUSATI ASHTIANI F.

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Journal: Iranian Journal of Basic Medical Sciences Year:2003 | Volume:5 | Issue:4 (16) Page(s): 216-225

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Title

APOPTOTIC EFFECT OF TAMOXIFEN IN THE INFILTRATING BREAST ADENOCARCINOMA MDA-MB468 CELLS: THE INVOLVEMENT OF CASPASE-3, BCL-2 AND BAX PROTEINS

Author(s)

KARAMI TEHRANI FATEMEH | SALAMI S. | OUSATI ASHTIANI F. | Issue Writer Certificate

Keywords

APOPTOSISQ1

BAXQ4

BCL-2Q2

CASPASE-3Q4

MDA-MB-468 CELL LINEQ4

TAMOXIFENQ4

Abstract

Investigations on the mode of action of TAMOXIFEN in the estrogen receptor negative tumors have continued at cellular and molecular levels mainly because of its observed efficacy in estrogen receptor negative cancers. Therefore, this study has been proposed to examine the effect of TAMOXIFEN on the induction of APOPTOSIS in estrogen receptor negative breast cancer cell line (MDA-MB-468) and to scrutinize the role of CASPASE-3, BCL-2 and BAX proteins. Proliferation and APOPTOSIS were investigated by flow cytometry and Hoechst 33258 staining methods. A caspases-3 colorimetric assay kit was used to investigate casp 3 activation. The levels of BCL-2 and BAX proteins were obtained using western immunoblotting analysis. Results indicated that TAMOXIFEN, at high concentration (20 µM induced a significant (χ2 test, P<0.05) APOPTOSIS in a time dependent manner. The treated cells were elicited a time- and dose- dependent induction of CASPASE-3 activity (χ2 test, P<0.05). Pre-incubation of the cells with CASPASE-3 inhibitor (z- DEVD-fmk) blocked APOPTOSIS, implicating a critical role for caspase cascade. The western immunoblotting analysis revealed no marked alterations in BCL-2 and BAX, indicating a non-mithconderial apoptotic pathway. Since an induction of CASPASE-3 dependent APOPTOSIS was observed in TAMOXIFEN treated estrogen receptor negative breast cancer cells, it is concluded that it could be used as an effective therapeutic agent against estrogen independent cancers.

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APA: Copy

KARAMI TEHRANI, FATEMEH, SALAMI, S., & OUSATI ASHTIANI, F.. (2003). APOPTOTIC EFFECT OF TAMOXIFEN IN THE INFILTRATING BREAST ADENOCARCINOMA MDA-MB468 CELLS: THE INVOLVEMENT OF CASPASE-3, BCL-2 AND BAX PROTEINS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 5(4 (16)), 216-225. SID. https://sid.ir/paper/52363/en

Vancouver: Copy

KARAMI TEHRANI FATEMEH, SALAMI S., OUSATI ASHTIANI F.. APOPTOTIC EFFECT OF TAMOXIFEN IN THE INFILTRATING BREAST ADENOCARCINOMA MDA-MB468 CELLS: THE INVOLVEMENT OF CASPASE-3, BCL-2 AND BAX PROTEINS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2003;5(4 (16)):216-225. Available from: https://sid.ir/paper/52363/en

IEEE: Copy

FATEMEH KARAMI TEHRANI, S. SALAMI, and F. OUSATI ASHTIANI, “APOPTOTIC EFFECT OF TAMOXIFEN IN THE INFILTRATING BREAST ADENOCARCINOMA MDA-MB468 CELLS: THE INVOLVEMENT OF CASPASE-3, BCL-2 AND BAX PROTEINS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 5, no. 4 (16), pp. 216–225, 2003, [Online]. Available: https://sid.ir/paper/52363/en

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