SCREENING AND ISOLATION OF CEPHALOSPORIN C-PRODUCING STRAINS, PRODUCTION AND PURIFICATION OF ANTIBIOTIC

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MOAZAMI N.; MIRDAMADI S.; KHAN MOHAMMADI M.; AZARNOUSH MORTEZA

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Journal Paper

Paper Information

Journal: Feyz Medical Sciences Journal Year:2003 | Volume:6 | Issue:23 Page(s): 0-0

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Title

SCREENING AND ISOLATION OF CEPHALOSPORIN C-PRODUCING STRAINS, PRODUCTION AND PURIFICATION OF ANTIBIOTIC

Author(s)

MOAZAMI N. | MIRDAMADI S. | KHAN MOHAMMADI M. | AZARNOUSH MORTEZA | Issue Writer Certificate

Keywords

CEPHALOSPORIN C

ACROMONIUM

SCREENING

PURIFICATION

Abstract

Background: CEPHALOSPORIN C (CPC), as a major precursor of semisynthetic cephalosporin antibiotics, is produced by species of ACROMONIUM genus in certain conditions. Thus the present study was carried out to isolate and obtain the strains and detecting their production as well as defining optimum production and PURIFICATION conditions. Materials and methods: During this exploratory study, selective media were used for soil SCREENING program. Standard strains were purchased from DSMZ (Germany). CPC production was confirmed by biological and chromatographic (paper chromatography and HPLC) techniques. To produce induction, different kinds of media with various carbon and nitrogen sources were used. CPC were removed from filtered and acid-treated broth by absorption on a neutral macroporous resin (XAD-4), followed by absorption on a weakly basic ion-exchange resin (IRA-67). Results: In soil SCREENING program, a native CPC-producing strain was isolated. CPC production of this strain and also of standard strains was confirmed by other methods. Native and standard strains were differed in CPC production in various fermentation media. CPC was recovered and extracted from these media with acceptable purity. Conclusion: Results have implied that this product can be used for large-scale production of CPC in our country

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APA: Copy

MOAZAMI, N., MIRDAMADI, S., KHAN MOHAMMADI, M., & AZARNOUSH, MORTEZA. (2003). SCREENING AND ISOLATION OF CEPHALOSPORIN C-PRODUCING STRAINS, PRODUCTION AND PURIFICATION OF ANTIBIOTIC. FEYZ, 6(23), 0-0. SID. https://sid.ir/paper/532194/en

Vancouver: Copy

MOAZAMI N., MIRDAMADI S., KHAN MOHAMMADI M., AZARNOUSH MORTEZA. SCREENING AND ISOLATION OF CEPHALOSPORIN C-PRODUCING STRAINS, PRODUCTION AND PURIFICATION OF ANTIBIOTIC. FEYZ[Internet]. 2003;6(23):0-0. Available from: https://sid.ir/paper/532194/en

IEEE: Copy

N. MOAZAMI, S. MIRDAMADI, M. KHAN MOHAMMADI, and MORTEZA AZARNOUSH, “SCREENING AND ISOLATION OF CEPHALOSPORIN C-PRODUCING STRAINS, PRODUCTION AND PURIFICATION OF ANTIBIOTIC,” FEYZ, vol. 6, no. 23, pp. 0–0, 2003, [Online]. Available: https://sid.ir/paper/532194/en

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