IDENTIFICATION OF CANDIDA SPECIES AMONG PATIENTS WITH VULVOVAGINAL CANDIDIASIS IN KASHAN BY PCR-RFLP METHOD

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MOHAMMADI RASOUL; NAZERI MEHDI; MESDAGHINIA ELAHEH; MIRHENDI SEYED HOSSEIN

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Journal Paper

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Journal: Journal of Isfahan Medical School Year:2012 | Volume:29 | Issue:165 Page(s): 0-0

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Title

IDENTIFICATION OF CANDIDA SPECIES AMONG PATIENTS WITH VULVOVAGINAL CANDIDIASIS IN KASHAN BY PCR-RFLP METHOD

Author(s)

MOHAMMADI RASOUL | NAZERI MEHDI | MESDAGHINIA ELAHEH | MIRHENDI SEYED HOSSEIN | Issue Writer Certificate

Keywords

CANDIDA

VULVOVAGINITIS

PCR-RFLP

Abstract

Background: Vulvovaginal candidiasis (VVC) is a common vaginal infection, affecting up to 75% of women in child-bearing age. Approximately 5% of patients may experience recurrent VVC. CANDIDA albicansis the most common causative agent with a prevalence of approximately 70–90%. Nonalbicans CANDIDA species, predominantly CANDIDA glabrata, are responsible for the remainder. Precise diagnosis of causative agents of VVC is necessary for epidemiological purposes and for effective treatment.Methods: One hundred and thirty three yeast isolates were collected from a gynecology clinic affiliated to Kashan University, Iran, during 2007-2009. Genomic DNA of each isolate was extracted from fresh cultures using FTA-card. The ITS1-ITS2 region was amplified by polymerase chain reaction (PCR) assay and was digested by the restriction enzymeHpaII. The products of restriction fragment length polymorphism (RFLP) were electrophoresed on agarose gel and the yeast species were determined according to differences in their electrophoretic band patterns.Findings: Prominent species isolated from clinical specimens were CANDIDA albinos responsible for 116 cases (87.2%), followed by 16 cases of C. glabrata (12%) and 1 case of C. kefyr (0.7%). The age bracket of 31-40 years had the highest and the age bracket of 11-20 years had the lowest frequency of occurrence.Conclusion: Like the majority of similar studies performed in this field, the present study found CANDIDA albicansas predominant species isolated from vulvovaginal candidiasis. As molecular diagnostic methods are rapid and reliable and as there are no any other studies that used PCR-RFLP for diagnosis of etiologic agents of VVC in Iran, we recommend this approach for other similar epidemiological studies.

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APA: Copy

MOHAMMADI, RASOUL, NAZERI, MEHDI, MESDAGHINIA, ELAHEH, & MIRHENDI, SEYED HOSSEIN. (2012). IDENTIFICATION OF CANDIDA SPECIES AMONG PATIENTS WITH VULVOVAGINAL CANDIDIASIS IN KASHAN BY PCR-RFLP METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 29(165), 0-0. SID. https://sid.ir/paper/592515/en

Vancouver: Copy

MOHAMMADI RASOUL, NAZERI MEHDI, MESDAGHINIA ELAHEH, MIRHENDI SEYED HOSSEIN. IDENTIFICATION OF CANDIDA SPECIES AMONG PATIENTS WITH VULVOVAGINAL CANDIDIASIS IN KASHAN BY PCR-RFLP METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2012;29(165):0-0. Available from: https://sid.ir/paper/592515/en

IEEE: Copy

RASOUL MOHAMMADI, MEHDI NAZERI, ELAHEH MESDAGHINIA, and SEYED HOSSEIN MIRHENDI, “IDENTIFICATION OF CANDIDA SPECIES AMONG PATIENTS WITH VULVOVAGINAL CANDIDIASIS IN KASHAN BY PCR-RFLP METHOD,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 29, no. 165, pp. 0–0, 2012, [Online]. Available: https://sid.ir/paper/592515/en

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