THREE-DIMENSIONAL SCAFFOLD FROM DECELLULARIZED HUMAN GINGIVA FOR CELL CULTURES: GLYCOCONJUGATES AND CELL BEHAVIOR

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NADERI SOMAYEH; KHAYAT ZADEH JINA; MAHDAVI SHAHRI NASSER; NEJAD SHAHROKH ABADI KHADIJEH; CHERAVI MOJTABA; BAHARARA JAVAD; BANIHASHEMRAD SEYEDALI; BAHRAMI AHMAD REZA

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Journal: Cell Journal (Yakhteh) Year:2013 | Volume:15 | Issue:2 (58) Page(s): 166-175

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Title

THREE-DIMENSIONAL SCAFFOLD FROM DECELLULARIZED HUMAN GINGIVA FOR CELL CULTURES: GLYCOCONJUGATES AND CELL BEHAVIOR

Author(s)

Keywords

BLASTEMA TISSUE

SCAFFOLD

GINGIVA

DECELLULARIZATION

EXTRACELLULAR MATRIX

Abstract

Objective: We studied both the presence of some carbohydrate compounds in a three-dimensional (3D) matrix harvested from human GINGIVA and the cell behavior in this matrix.Materials and Methods: In this experimental research, in order to prepare 3D SCAFFOLDs, human palatal GINGIVAl biopsies were harvested and physically decellularized by freeze-thawing and sodium dodecyl sulfate (SDS). The SCAFFOLDs were placed within the rings of BLASTEMA TISSUEs obtained from a pinna rabbit, in vitro. We evaluated the presence of glycoconjugatesand cellular behavior according to histological, histochemical and spectrophotometry techniques at one, two and three weeks after culture. One-way analysis of variance (ANOVA) comparedthe groups.Results: EXTRACELLULAR MATRIX (ECM) remained after DECELLULARIZATION of tissue with 1% SDS. Glycoconjugate contents decreased meaningfully at a higher SDS concentration (p<0.0001). After culture of the ECM SCAFFOLD with blastema, we observed increased staining of alcian blue, periodic acid-Schiff (PAS) and toluidine blue in the SCAFFOLD and a number of other migrant cells which was caused by cell penetrationinto the SCAFFOLD. Spectrophotometry results showed an increase in glycosaminoglycans (GAGs) of the decellularized SCAFFOLDs at three weeks after culture.Conclusion: The present study has shown that a SCAFFOLD generated from palatal GINGIVAl tissue ECM is a suitable substrate for blastema cell migration and activity. This SCAFFOLD maypotentially be useful as a biological SCAFFOLD in tissue engineering applications.

Cites

PREPARATION OF HUMAN 3D SKIN MATRIX AND INVESTIGATION OF THE INTERACTION BETWEEN RAT ADHERENT BONE MARROW CELLS AND PREPARED MATRIX

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APA: Copy

NADERI, SOMAYEH, KHAYAT ZADEH, JINA, MAHDAVI SHAHRI, NASSER, NEJAD SHAHROKH ABADI, KHADIJEH, CHERAVI, MOJTABA, BAHARARA, JAVAD, BANIHASHEMRAD, SEYEDALI, & BAHRAMI, AHMAD REZA. (2013). THREE-DIMENSIONAL SCAFFOLD FROM DECELLULARIZED HUMAN GINGIVA FOR CELL CULTURES: GLYCOCONJUGATES AND CELL BEHAVIOR. CELL JOURNAL (YAKHTEH), 15(2 (58)), 166-175. SID. https://sid.ir/paper/598792/en

Vancouver: Copy

NADERI SOMAYEH, KHAYAT ZADEH JINA, MAHDAVI SHAHRI NASSER, NEJAD SHAHROKH ABADI KHADIJEH, CHERAVI MOJTABA, BAHARARA JAVAD, BANIHASHEMRAD SEYEDALI, BAHRAMI AHMAD REZA. THREE-DIMENSIONAL SCAFFOLD FROM DECELLULARIZED HUMAN GINGIVA FOR CELL CULTURES: GLYCOCONJUGATES AND CELL BEHAVIOR. CELL JOURNAL (YAKHTEH)[Internet]. 2013;15(2 (58)):166-175. Available from: https://sid.ir/paper/598792/en

IEEE: Copy

SOMAYEH NADERI, JINA KHAYAT ZADEH, NASSER MAHDAVI SHAHRI, KHADIJEH NEJAD SHAHROKH ABADI, MOJTABA CHERAVI, JAVAD BAHARARA, SEYEDALI BANIHASHEMRAD, and AHMAD REZA BAHRAMI, “THREE-DIMENSIONAL SCAFFOLD FROM DECELLULARIZED HUMAN GINGIVA FOR CELL CULTURES: GLYCOCONJUGATES AND CELL BEHAVIOR,” CELL JOURNAL (YAKHTEH), vol. 15, no. 2 (58), pp. 166–175, 2013, [Online]. Available: https://sid.ir/paper/598792/en

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