مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

370
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

198
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

3

Information Journal Paper

Title

AUTOLOGOUS TRANSPLANTATION OF ADULT MICE SPERMATOGONIAL STEM CELLS INTO GAMMA IRRADIATED TESTES

Pages

  82-89

Abstract

 Objective: We evaluated structural and functional changes of fresh and frozen-thawed adult mouse spermatogonial stem cells following auto-transplantation into gamma-irra diated testes.Materials and Methods: In this experimental research, the right testes from adult mice (n=25) were collected, then Sertoli and spermatogonial cells were isolated using two-step enzymatic digestion, lectin immobilization and differential plating.Three weeks after culti vation, the Bromodeoxyuridine (BrdU) -labeled spermatogonial cells were transplanted, via rete testis, into the other testis of the same mouse, which had been irradiated with 14Gy. The mice were transplanted with: fresh cells (control 1), fresh cells co-cultured with Ser toli cells (control 2), the frozen-thawed cells (experimental 1) and frozen-thawed cells co-cultured with Sertoli cells (experimental 2). The morphological changes between different transplanted testes groups were compared in 8 weeks after transplantation. The statistical significance between mean values was determined by Kruskal Wallis and one-way analysis of variance in efficiency of transplantation.Results: The statistical analysis revealed significant increases in the mean percentage of testis weight and normal seminiferous tubules following spermatogonial stem cells trans plantation in the recipient’s testes. The normal seminiferous tubules percentage in the co-culture system with fresh cells and frozen-thawed groups were more than those in non-transplanted and fresh cell transplanted groups (p£0.001).Conclusion: Our results demonstrated that spermatogonial stem cells in the colonies could result sperm production in the recipient’s testes after autologous transplantation.

Cites

References

  • No record.

    • Cite

    APA: Copy

    KORUJI, MORTEZA, MOVAHEDIN, MANSOUREH, MOWLA, SEYED JAVAD, GOURABI, HAMID, POURBEIRANVAND, SHAHRAM, & JABBARI ARFAEE, ALI. (2012). AUTOLOGOUS TRANSPLANTATION OF ADULT MICE SPERMATOGONIAL STEM CELLS INTO GAMMA IRRADIATED TESTES. CELL JOURNAL (YAKHTEH), 14(2 (54)), 82-89. SID. https://sid.ir/paper/600565/en

    Vancouver: Copy

    KORUJI MORTEZA, MOVAHEDIN MANSOUREH, MOWLA SEYED JAVAD, GOURABI HAMID, POURBEIRANVAND SHAHRAM, JABBARI ARFAEE ALI. AUTOLOGOUS TRANSPLANTATION OF ADULT MICE SPERMATOGONIAL STEM CELLS INTO GAMMA IRRADIATED TESTES. CELL JOURNAL (YAKHTEH)[Internet]. 2012;14(2 (54)):82-89. Available from: https://sid.ir/paper/600565/en

    IEEE: Copy

    MORTEZA KORUJI, MANSOUREH MOVAHEDIN, SEYED JAVAD MOWLA, HAMID GOURABI, SHAHRAM POURBEIRANVAND, and ALI JABBARI ARFAEE, “AUTOLOGOUS TRANSPLANTATION OF ADULT MICE SPERMATOGONIAL STEM CELLS INTO GAMMA IRRADIATED TESTES,” CELL JOURNAL (YAKHTEH), vol. 14, no. 2 (54), pp. 82–89, 2012, [Online]. Available: https://sid.ir/paper/600565/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button