ISOLATION OF CD34 POSITIVE HAIR FOLLICLE STEM CELLS USING MAGNETIC ACTIVATING CELL SORTING (MACS) METHOD

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HEYDARI TAJADDOD SH.; NAJAFZADEH N.; MAHDAVI RAD M.; SHEIKHKANLOUI MILAN H.; KALARESTAGHI H.; NEJATI V.

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Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2015 | Volume:15 | Issue:2 Page(s): 171-178

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Title

ISOLATION OF CD34 POSITIVE HAIR FOLLICLE STEM CELLS USING MAGNETIC ACTIVATING CELL SORTING (MACS) METHOD

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Keywords

HAIR FOLLICLE STEM CELLSQ1

MAGNETIC ACTIVATING CELL SORTINGQ2

CD34Q2

FLUORESCENT MICROSCOPYQ2

Abstract

Background & objectives: HAIR FOLLICLE STEM CELLS (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used MAGNETIC ACTIVATING CELL SORTING (MACS) to separate HFSCs.Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a FLUORESCENT MICROSCOPY.Results: Here, we isolated CD34 positive cells using MACS and 12±1.04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.

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APA: Copy

HEYDARI TAJADDOD, SH., NAJAFZADEH, N., MAHDAVI RAD, M., SHEIKHKANLOUI MILAN, H., KALARESTAGHI, H., & NEJATI, V.. (2015). ISOLATION OF CD34 POSITIVE HAIR FOLLICLE STEM CELLS USING MAGNETIC ACTIVATING CELL SORTING (MACS) METHOD. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 15(2 ), 171-178. SID. https://sid.ir/paper/60069/en

Vancouver: Copy

HEYDARI TAJADDOD SH., NAJAFZADEH N., MAHDAVI RAD M., SHEIKHKANLOUI MILAN H., KALARESTAGHI H., NEJATI V.. ISOLATION OF CD34 POSITIVE HAIR FOLLICLE STEM CELLS USING MAGNETIC ACTIVATING CELL SORTING (MACS) METHOD. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2015;15(2 ):171-178. Available from: https://sid.ir/paper/60069/en

IEEE: Copy

SH. HEYDARI TAJADDOD, N. NAJAFZADEH, M. MAHDAVI RAD, H. SHEIKHKANLOUI MILAN, H. KALARESTAGHI, and V. NEJATI, “ISOLATION OF CD34 POSITIVE HAIR FOLLICLE STEM CELLS USING MAGNETIC ACTIVATING CELL SORTING (MACS) METHOD,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 15, no. 2 , pp. 171–178, 2015, [Online]. Available: https://sid.ir/paper/60069/en

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