STUDY OF RELATIONSHIP BETWEEN GENETIC PATTERN AND SUSCEPTIBILITY TO FLUCONAZOLE IN CLINICAL ISOLATED OF TRICHOPHYTON RUBRUM

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HADADI F.; SABOKBAR A.; DEZFULIAN M.; BAKHTIARI A.

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Journal: Journal of Ardabil University of Medical Sciences Year:2015 | Volume:15 | Issue:2 Page(s): 154-170

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Title

STUDY OF RELATIONSHIP BETWEEN GENETIC PATTERN AND SUSCEPTIBILITY TO FLUCONAZOLE IN CLINICAL ISOLATED OF TRICHOPHYTON RUBRUM

Author(s)

HADADI F. | SABOKBAR A. | DEZFULIAN M. | BAKHTIARI A. | Issue Writer Certificate

Keywords

TRICHOPHYTON RUBRUMQ2

FLUCONAZOLEQ3

RAPD-PCRQ2

GENETIC DIVERSITYQ2

Abstract

Background & objectives: TRICHOPHYTON RUBRUM isone of the most common pathogenic causes of dermatophytosis. One of the drugs prescribed for fungal infections is FLUCONAZOLE which belongs to Azoles group of antifungal agents. Recently molecular typing methods have been developed for answering the epidemiological questions and disease recurrence problems. Current study has been conducted on 22 isolates ofTRICHOPHYTON RUBRUM obtained from patients randomly. Our aim was the investigation of correlation between genetic pattern and sensitivity to FLUCONAZOLE in clinical isolates ofTRICHOPHYTON RUBRUM.Methods: Firstly the genus and species of isolated fungi from patients have been confirmed by macroscopic and microscopic methods. Then, the resistance and sensitivity of isolates against drug have been determined using culture medium containing defined amount of drug. In next step fungal DNA has been extracted by RAPD-PCR (random amplified polymorphic DNA) with random sequences of 3 primers.Results: Each primer produced different amplified pattern, and differences have been observed in genetic pattern of resistant and sensitive samples using each 3 primers, but there was no bond with 100% specificity.Conclusion: The 12 sensitive isolates which didn’t grow in 50μg/ml concentration of drug, also had limited growth at the lower concentration of drug. Ten resistant isolates which grew in 50μg/ml of drug, also showed resistant to lower concentration of drug. There are differences in genetic pattern of resistant and sensitive samples. RAPD analysis for molecular typing of TRICHOPHYTON RUBRUMseems to be completely suitable.

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APA: Copy

HADADI, F., SABOKBAR, A., DEZFULIAN, M., & BAKHTIARI, A.. (2015). STUDY OF RELATIONSHIP BETWEEN GENETIC PATTERN AND SUSCEPTIBILITY TO FLUCONAZOLE IN CLINICAL ISOLATED OF TRICHOPHYTON RUBRUM. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 15(2 ), 154-170. SID. https://sid.ir/paper/60077/en

Vancouver: Copy

HADADI F., SABOKBAR A., DEZFULIAN M., BAKHTIARI A.. STUDY OF RELATIONSHIP BETWEEN GENETIC PATTERN AND SUSCEPTIBILITY TO FLUCONAZOLE IN CLINICAL ISOLATED OF TRICHOPHYTON RUBRUM. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2015;15(2 ):154-170. Available from: https://sid.ir/paper/60077/en

IEEE: Copy

F. HADADI, A. SABOKBAR, M. DEZFULIAN, and A. BAKHTIARI, “STUDY OF RELATIONSHIP BETWEEN GENETIC PATTERN AND SUSCEPTIBILITY TO FLUCONAZOLE IN CLINICAL ISOLATED OF TRICHOPHYTON RUBRUM,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 15, no. 2 , pp. 154–170, 2015, [Online]. Available: https://sid.ir/paper/60077/en

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