CLONING OF 1183 BP FRAGMENT FROM RHOPTRY PROTEIN I (ROPI) GENE OF TOXOPLASMA GONDII (RH) IN EXPRESSION PROKARYOTE PLASMID PET32A

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ESLAMI RAD ZAHRA; GHORBANZADEH BEHZAD; HAJI HOSSEINI REZA; ABTAHI HAMID; MOSAYEBI MAHDI; SHOJAEI SAEIDEH; SADEGHI ABDOLRAHIM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Zahedan Journal of Research in Medical Sciences Year:2013 | Volume:15 | Issue:10 Page(s): 32-36

Download Full-Text

View:

271

Download:

119

Cites:

Information Journal Paper

Title

CLONING OF 1183 BP FRAGMENT FROM RHOPTRY PROTEIN I (ROPI) GENE OF TOXOPLASMA GONDII (RH) IN EXPRESSION PROKARYOTE PLASMID PET32A

Author(s)

Keywords

CLONING PROKARYOTIC PLASMID RHOPTRY PROTEIN I (ROP I) TOXOPLASMA GONDII

Abstract

Background: Toxoplasma gondii is an obligatory intracellular protozoan. Considering to high prevalence of this disease the best way to reduce the raised loses is prevention of human and animal infection, rapid diagnosis, differentiation between acute and chronic disease. Rhoptry protein 1 of Toxoplasma gondii is an excretory-secretory antigen that exists in the most stages of life cycle. According to specifications of excretory-secretory antigen that seems this antigen is a suitable candidate to produce recombinant vaccine and diagnostic kit. The main object of the present work was cloning rhoptry protein 1 (ROP1) gene of Toxoplasma gondii (RH) in a cloning vector for further production of rhoptry proteins.Materials and Methods: Genomic DNA was extracted by phenol-chloroform method. The ROP1 fragment was amplified by PCR. This product was approved by sequencing and was cloned between the EcoR1 and Sal1 sites of the pTZ57R/T vector. Then transformed into Escherichia coli DH5α strain and screened by IPTG and X-Gal. After isolating of this gene from pTZ57R/T, it was subcloned into pET32a plasmid.Results: The plasmid was purified and approved by electrophoresis, enzyme restriction and PCR. After isolating of this gene from pTZ57R/T, it was subcloned into pET32a plasmid. After enzyme restriction and electrophoresis a fragment about 1183bp was separated from pET32a.Conclusion: Recombinant plasmid of ROP1 gene was constructed and ready for future study. That seems the antigen is a suitable candidate to produce recombinant vaccine and diagnostic kit.

Cites

No record.

References

No record.

Cite

APA: Copy

ESLAMI RAD, ZAHRA, GHORBANZADEH, BEHZAD, HAJI HOSSEINI, REZA, ABTAHI, HAMID, MOSAYEBI, MAHDI, SHOJAEI, SAEIDEH, & SADEGHI, ABDOLRAHIM. (2013). CLONING OF 1183 BP FRAGMENT FROM RHOPTRY PROTEIN I (ROPI) GENE OF TOXOPLASMA GONDII (RH) IN EXPRESSION PROKARYOTE PLASMID PET32A. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 15(10), 32-36. SID. https://sid.ir/paper/605817/en

Vancouver: Copy

ESLAMI RAD ZAHRA, GHORBANZADEH BEHZAD, HAJI HOSSEINI REZA, ABTAHI HAMID, MOSAYEBI MAHDI, SHOJAEI SAEIDEH, SADEGHI ABDOLRAHIM. CLONING OF 1183 BP FRAGMENT FROM RHOPTRY PROTEIN I (ROPI) GENE OF TOXOPLASMA GONDII (RH) IN EXPRESSION PROKARYOTE PLASMID PET32A. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2013;15(10):32-36. Available from: https://sid.ir/paper/605817/en

IEEE: Copy

ZAHRA ESLAMI RAD, BEHZAD GHORBANZADEH, REZA HAJI HOSSEINI, HAMID ABTAHI, MAHDI MOSAYEBI, SAEIDEH SHOJAEI, and ABDOLRAHIM SADEGHI, “CLONING OF 1183 BP FRAGMENT FROM RHOPTRY PROTEIN I (ROPI) GENE OF TOXOPLASMA GONDII (RH) IN EXPRESSION PROKARYOTE PLASMID PET32A,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 15, no. 10, pp. 32–36, 2013, [Online]. Available: https://sid.ir/paper/605817/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops