مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

GENETIC PROPERTIES OF BLACTX-M AND BLAPER β-LACTAMASE GENES IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE BY POLYMERASE CHAIN REACTION

Pages

  378-383

Abstract

 Objective (s): blaCTX-M and blaPER are two genes that encode class A extended-spectrum b-lactamases (ESBLs) and can be responsible for therapeutic problems. This study was carried out to evaluate the molecular properties of these genes in clinical isolates of ENTEROBACTERIACEAE by polymerase chain reaction (PCR), restriction digestion and sequencing.Materials and Methods: During six months, starting from January 2012, one hundred clinical isolates of ENTEROBACTERIACEAE were collected from urinary samples. The ESBL-producing isolates were detected by phenotypic confirmation test. After plasmid extraction, blaPER and blaCTX-M genes were detected using PCR by specific primers. The blaCTX-M PCR products were digested with Taq1, and two of the blaCTX-M genes were sequenced.Results: Phenotypic tests showed that 27 (27%) isolates were ESBL producers with the highest frequency for Klebsiella pneumoniae (47.4%) and Escherichia coli (17.9%). Twenty six (26%) of ENTEROBACTERIACEAE isolates harbored the blaCTX-M gene, and none of them had blaPER. The restriction analysis of PCR products showed that all blaCTX-M amplified products had the same patterns. Both sequenced bacteria were CTX-M-15 type ESBL carriers.Conclusion: The results of this study showed the blaCTX-M-15 gene in ENTEROBACTERIACEAE isolates for the first time in Mashhad, Iran. High degrees of associated resistance to co-trimoxazole and gentamicin were found in ESBL producers. Therefore, an integrated and regular management of antibiotic prescription need to be trained in our society.

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    APA: Copy

    NAKHAEI MOGHADDAM, MAHBOOBEH, HASHEMI BEIDOKHTI, MEHRDAD, AMEL JAMEHDAR, SAEID, & GHAHRAMAN, MARTHA. (2014). GENETIC PROPERTIES OF BLACTX-M AND BLAPER β-LACTAMASE GENES IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE BY POLYMERASE CHAIN REACTION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 17(5), 378-383. SID. https://sid.ir/paper/629238/en

    Vancouver: Copy

    NAKHAEI MOGHADDAM MAHBOOBEH, HASHEMI BEIDOKHTI MEHRDAD, AMEL JAMEHDAR SAEID, GHAHRAMAN MARTHA. GENETIC PROPERTIES OF BLACTX-M AND BLAPER β-LACTAMASE GENES IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE BY POLYMERASE CHAIN REACTION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2014;17(5):378-383. Available from: https://sid.ir/paper/629238/en

    IEEE: Copy

    MAHBOOBEH NAKHAEI MOGHADDAM, MEHRDAD HASHEMI BEIDOKHTI, SAEID AMEL JAMEHDAR, and MARTHA GHAHRAMAN, “GENETIC PROPERTIES OF BLACTX-M AND BLAPER β-LACTAMASE GENES IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE BY POLYMERASE CHAIN REACTION,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 17, no. 5, pp. 378–383, 2014, [Online]. Available: https://sid.ir/paper/629238/en

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