EVALUATION OF THE EFFECTS OF ASCORBIC ACID ON GLYCATION REACTION OF ALBUMIN ON IN VITRO CONDITION

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MEAMAR ZEINAB; MASOUDIAN NAHID; BAHMANI FERESHTEH

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Journal: Studies in Medical Sciences Year:2017 | Volume:28 | Issue:5 Page(s): 363-372

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Title

EVALUATION OF THE EFFECTS OF ASCORBIC ACID ON GLYCATION REACTION OF ALBUMIN ON IN VITRO CONDITION

Author(s)

MEAMAR ZEINAB | MASOUDIAN NAHID | BAHMANI FERESHTEH | Issue Writer Certificate

Keywords

GLYCATIONQ1

DIABETESQ1

ADVANCED GLYCATION END PRODUCTSQ1

ASCORBIC ACIDQ2

ALBUMINQ2

Abstract

Background & Aims: Advanced GLYCATION end products (AGEs) formation is increased in DIABETES mellitus, leading to micro vascular and macro vascular complications. Recently, much attention has been focused on natural and synthetic inhibitors to delay the onset or progression of DIABETES and its comorbidities. In this study, an in vitro GLYCATION model containing ALBUMIN as a model protein together with glucose as glycating agent was used to study antiGLYCATION activity of AA.Materials & Methods: Bovine serum ALBUMIN was incubated with 0.5 M of glucose with or without AA in 37°C for 4 weeks. The formation of fluorescent AGEs was determined to indicate protein GLYCATION, whereas the level of protein carbonyl content and thiol group were examined for protein oxidation. GLYCATION is known to induce aggregation and fibrillation of proteins. To determine the inhibitory effect of AA on aggregation and fibrillation of ALBUMIN, amyloid specific dyes such as Thioflavin T was used.Results: The results found that AA significantly inhibited the formation of fluorescent AGEs (P<0.05) and significantly prevented protein oxidation manifested by reducing protein carbonyl and the depletion of protein thiol groups (P<0.05). Moreover, our results indicated the inhibitory potential of AA toward amyloid like aggregation of ALBUMIN and protective effect of AA on ALBUMIN native conformation.Conclusion: Thus, these findings indicated that AA has high potential for decreasing protein GLYCATION and oxidation that may delay or prevent AGEs-related diabetic complications.

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References

GLYCATED ALBUMIN: AN OVERVIEW OF THE IN VITRO MODELS OF AN IN VIVO POTENTIAL DISEASE MARKER

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APA: Copy

MEAMAR, ZEINAB, MASOUDIAN, NAHID, & BAHMANI, FERESHTEH. (2017). EVALUATION OF THE EFFECTS OF ASCORBIC ACID ON GLYCATION REACTION OF ALBUMIN ON IN VITRO CONDITION. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 28(5), 363-372. SID. https://sid.ir/paper/63675/en

Vancouver: Copy

MEAMAR ZEINAB, MASOUDIAN NAHID, BAHMANI FERESHTEH. EVALUATION OF THE EFFECTS OF ASCORBIC ACID ON GLYCATION REACTION OF ALBUMIN ON IN VITRO CONDITION. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2017;28(5):363-372. Available from: https://sid.ir/paper/63675/en

IEEE: Copy

ZEINAB MEAMAR, NAHID MASOUDIAN, and FERESHTEH BAHMANI, “EVALUATION OF THE EFFECTS OF ASCORBIC ACID ON GLYCATION REACTION OF ALBUMIN ON IN VITRO CONDITION,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 28, no. 5, pp. 363–372, 2017, [Online]. Available: https://sid.ir/paper/63675/en

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