MOLECULAR ANALYSIS AND COMPARISON OF Y CHROMOSOME MICRODELETIONS IN TABRIZ AND KASHAN INFERTILE MEN WITH AZOOSPERMIA AND SEVERE OLIGOSPERMIA

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TORFEH MAHNAZ; SAKHINIA EBRAHIM; HASANI HASAN; AHMADI ASRE BADR YADOLLAH; NOURIZADEH DAVOUD; HESHMAT YAGHOUB; ROHANI MAHDI

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Paper Information

Journal: Iranian Journal of Obstetrics, Gynecology and Infertility Year:2012 | Volume:15 | Issue:2 Page(s): 23-30

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Title

MOLECULAR ANALYSIS AND COMPARISON OF Y CHROMOSOME MICRODELETIONS IN TABRIZ AND KASHAN INFERTILE MEN WITH AZOOSPERMIA AND SEVERE OLIGOSPERMIA

Author(s)

Keywords

INFERTILITYQ3

MICRO DELETIONSQ2

Y CHROMOSOMEQ2

MOLECULAR ANALYSISQ2

AZOOSPERMIAQ2

OLIGOSPERMIAQ2

INFERTILE MENQ2

Abstract

Introduction: Studies has shown that approximately 10-15% of married couples have INFERTILITY problem and a male factor can be accounted for half of these cases. Deletion in three non- overlapping regions on long arm of Y CHROMOSOME (AZFa, AZFb and AZFc) causes defective spermatogenesis and ultimately INFERTILITY in men. According to recent studies males who have Y CHROMOSOME deletions and they are by ICSI for INFERTILITY may transmit their deletions to a son offspring. So before ICSI all such men should undergo screening for the presence of these deletions in order to prevent the transmission to their male offspring.Methods: Screening of Y CHROMOSOME MICRO DELETIONS was done in 100 INFERTILE MEN who came to INFERTILITY center of Beheshti hospital in Kashan and INFERTILITY centers in Tabriz for ICSI. Genomic DNA was extracted from peripheral blood lymphocytes by standard ethanol chloroform techniques. By using 7 STS markers based on EAA/EMQN guideline and 11 STS markers which used in Iran and neighbor countries, PCR performed on each samples for detection of MICRO DELETIONS.Results: In Kashani participants, none of the patients had these deletions, but in Tabrizi patients 4 (8%) had these deletions.Conclusion: This study failed to find greatly deletion in the patients who referred for ICSI in our study, so it appears that other factors were important in the INFERTILITY in this population.

Cites

FREQUENCY OF Y CHROMOSOME MICRO DELETIONS AMONG IRANIAN INFERTILE MEN WITH AZOOSPERMIA AND SEVERE OLIGOZOOSPERMIA: A META-ANALYSIS

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APA: Copy

TORFEH, MAHNAZ, SAKHINIA, EBRAHIM, HASANI, HASAN, AHMADI ASRE BADR, YADOLLAH, NOURIZADEH, DAVOUD, HESHMAT, YAGHOUB, & ROHANI, MAHDI. (2012). MOLECULAR ANALYSIS AND COMPARISON OF Y CHROMOSOME MICRODELETIONS IN TABRIZ AND KASHAN INFERTILE MEN WITH AZOOSPERMIA AND SEVERE OLIGOSPERMIA. IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY, 15(2), 23-30. SID. https://sid.ir/paper/64503/en

Vancouver: Copy

TORFEH MAHNAZ, SAKHINIA EBRAHIM, HASANI HASAN, AHMADI ASRE BADR YADOLLAH, NOURIZADEH DAVOUD, HESHMAT YAGHOUB, ROHANI MAHDI. MOLECULAR ANALYSIS AND COMPARISON OF Y CHROMOSOME MICRODELETIONS IN TABRIZ AND KASHAN INFERTILE MEN WITH AZOOSPERMIA AND SEVERE OLIGOSPERMIA. IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY[Internet]. 2012;15(2):23-30. Available from: https://sid.ir/paper/64503/en

IEEE: Copy

MAHNAZ TORFEH, EBRAHIM SAKHINIA, HASAN HASANI, YADOLLAH AHMADI ASRE BADR, DAVOUD NOURIZADEH, YAGHOUB HESHMAT, and MAHDI ROHANI, “MOLECULAR ANALYSIS AND COMPARISON OF Y CHROMOSOME MICRODELETIONS IN TABRIZ AND KASHAN INFERTILE MEN WITH AZOOSPERMIA AND SEVERE OLIGOSPERMIA,” IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY, vol. 15, no. 2, pp. 23–30, 2012, [Online]. Available: https://sid.ir/paper/64503/en

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