MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES WITH HIGH COPY NUMBER OF IS6110 BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (SHORT COMMUNICATION)

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RAFIEE BEHNAM; MOSAVARI NADER; GHANI SEPIDEH; SADEGHI DAVOUD; KESHAVARZ ROUHOLAH; TADAYON KEYVAN

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Journal: Zahedan Journal of Research in Medical Sciences Year:2014 | Volume:16 | Issue:12 Page(s): 70-72

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Title

MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES WITH HIGH COPY NUMBER OF IS6110 BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (SHORT COMMUNICATION)

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Keywords

MYCOBACTERIUM BOVIS

RESTRICTION FRAGMENT LENGTH

POLYMORPHISM

TUBERCLUSIS

Abstract

Background: MYCOBACTERIUM BOVIS is the causative agent of bovine tuberculosis and belongs to the Mycobacterium tuberculosis complex. M. bovis usually carries only one or a few copies of the insertion sequence IS6110 in its genome. The aim of this study was evaluation of copy number of IS6110 in M. bovis isolates by RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) method.Materials and Methods: In this experimental study, 25 lymph node specimens of tuberculinpositive cattle were collected and cultured by standard methods, afterward genomic DNA was extracted by chloroform-isoamyl alcohol. Genetic studies were conducted by Pvull and DNA hybridization with IS6110.Results: Two isolates displayed more than of 4 copies of IS6110 by RFLP (IS6110-RFLP) method.Conclusion: The results of this study are unique and specific in Iran but reported in the world rarely. Therefore the new strains of M. bovis imported to Iran from other countries of the world.

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APA: Copy

RAFIEE, BEHNAM, MOSAVARI, NADER, GHANI, SEPIDEH, SADEGHI, DAVOUD, KESHAVARZ, ROUHOLAH, & TADAYON, KEYVAN. (2014). MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES WITH HIGH COPY NUMBER OF IS6110 BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (SHORT COMMUNICATION). ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 16(12), 70-72. SID. https://sid.ir/paper/665899/en

Vancouver: Copy

RAFIEE BEHNAM, MOSAVARI NADER, GHANI SEPIDEH, SADEGHI DAVOUD, KESHAVARZ ROUHOLAH, TADAYON KEYVAN. MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES WITH HIGH COPY NUMBER OF IS6110 BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (SHORT COMMUNICATION). ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2014;16(12):70-72. Available from: https://sid.ir/paper/665899/en

IEEE: Copy

BEHNAM RAFIEE, NADER MOSAVARI, SEPIDEH GHANI, DAVOUD SADEGHI, ROUHOLAH KESHAVARZ, and KEYVAN TADAYON, “MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES WITH HIGH COPY NUMBER OF IS6110 BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (SHORT COMMUNICATION),” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 16, no. 12, pp. 70–72, 2014, [Online]. Available: https://sid.ir/paper/665899/en

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