BORON INDUCES EARLY MATRIX MINERALIZATION VIA CALCIUM DEPOSITION AND ELEVATION OF ALKALINE PHOSPHATASE ACTIVITY IN DIFFERENTIATED RAT BONE MARROW MESENCHYMAL STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOVAHEDI NAJAFABADI BENT AL HODA; ABNOSI MOHAMMAD HUSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2016 | Volume:18 | Issue:1 (69) Page(s): 62-73

Download Full-Text

View:

409

Download:

196

Cites:

Information Journal Paper

Title

BORON INDUCES EARLY MATRIX MINERALIZATION VIA CALCIUM DEPOSITION AND ELEVATION OF ALKALINE PHOSPHATASE ACTIVITY IN DIFFERENTIATED RAT BONE MARROW MESENCHYMAL STEM CELLS

Author(s)

MOVAHEDI NAJAFABADI BENT AL HODA | ABNOSI MOHAMMAD HUSSEIN | Issue Writer Certificate

Keywords

BORIC ACID

MESENCHYMAL STEM CELLS

MORPHOLOGY

OSTEOBLASTS

Abstract

Objective: Boron (B) is essential for plant development and might be an essential micronutrient for animals and humans. This study was conducted to characterize the impact of BORIC ACID (BA) on the cellular and molecular nature of differentiated rat bone marrow MESENCHYMAL STEM CELLS (BMSCs).Materials and Methods: In this experimental study, BMSCs were extracted and expanded to the 3rd passage, then cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complemented with osteogenic media as well as 6 ng/ml and 6 mg/ml of BA. After 5, 10, 15 and 21 days the viability and the level of mineralization was determined using MTT assay and alizarin red respectively. In addition, the MORPHOLOGY, nuclear diameter and cytoplasmic area of the cells were studied with the help of fluorescent dye. The concentration of calcium, activity of alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) as well as sodium and potassium levels were also evaluated using commercial kits and a flame photometer respectively.Results: Although 6 mg/ml of BA was found to be toxic, a concentration of 6 ng/ml increased the osteogenic ability of the cell significantly throughout the treatment. In addition it was observed that B treatment caused the early induction of matrix mineralization compared to controls.Conclusion: Although more investigation is required, we suggest the prescription of a very low concentration of B in the form of BA or foods containing BA, in groups at high risk of osteoporosis or in the case of bone fracture.

Cites

No record.

References

Cite

APA: Copy

MOVAHEDI NAJAFABADI, BENT AL HODA, & ABNOSI, MOHAMMAD HUSSEIN. (2016). BORON INDUCES EARLY MATRIX MINERALIZATION VIA CALCIUM DEPOSITION AND ELEVATION OF ALKALINE PHOSPHATASE ACTIVITY IN DIFFERENTIATED RAT BONE MARROW MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 18(1 (69)), 62-73. SID. https://sid.ir/paper/676321/en

Vancouver: Copy

MOVAHEDI NAJAFABADI BENT AL HODA, ABNOSI MOHAMMAD HUSSEIN. BORON INDUCES EARLY MATRIX MINERALIZATION VIA CALCIUM DEPOSITION AND ELEVATION OF ALKALINE PHOSPHATASE ACTIVITY IN DIFFERENTIATED RAT BONE MARROW MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(1 (69)):62-73. Available from: https://sid.ir/paper/676321/en

IEEE: Copy

BENT AL HODA MOVAHEDI NAJAFABADI, and MOHAMMAD HUSSEIN ABNOSI, “BORON INDUCES EARLY MATRIX MINERALIZATION VIA CALCIUM DEPOSITION AND ELEVATION OF ALKALINE PHOSPHATASE ACTIVITY IN DIFFERENTIATED RAT BONE MARROW MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 18, no. 1 (69), pp. 62–73, 2016, [Online]. Available: https://sid.ir/paper/676321/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops