IN VITRO SUSCEPTIBILITY STUDY OF SALMONELLA TYPHIMURIUM MUTANT TO STRESS FACTORS

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AHANI AZARI ANIA; ZAHRAEI SALEHI TAGHI; NAYERI FASAEI BAHAR

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Journal Paper

Paper Information

Journal: MEDICAL LABORATORY JOURNAL Year:2016 | Volume:10 | Issue:6 Page(s): 28-31

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Information Journal Paper

Title

IN VITRO SUSCEPTIBILITY STUDY OF SALMONELLA TYPHIMURIUM MUTANT TO STRESS FACTORS

Author(s)

AHANI AZARI ANIA | ZAHRAEI SALEHI TAGHI | NAYERI FASAEI BAHAR | Issue Writer Certificate

Keywords

IN VITRO

MUTANT

SALMONELLA TYPHIMURIUM

DISEASE SUSCEPTIBILITY

Abstract

Background and Objective: In this study, we compared the susceptibility of SALMONELLA TYPHIMURIUM phoPmutant and its parent to stress conditions that the pathogen may encounter in a host.Methods: For this purpose, we used the phoP deletion MUTANT constructed in our previous study. In order to test the IN VITRO susceptibility of the MUTANT to stress factors, the effect of acidic pH, heat, bile salts and polymyxin on growth of the MUTANT was examined.Then, minimum inhibitory concentration and minimum bactericidal concentration of bile salts and polymyxin were determined.Salmonella typhimurium 14028 was used as the parent strain.Results: The MUTANT was highly susceptible to bile salts and polymyxin in comparison with the parent strain, but no difference was observed in their susceptibility to acid and heat.Conclusion: This study confirms the role of the phoP in resistance of Salmonella to polymyxin and bile salts. Mutation in thephoP leads to susceptibility of the MUTANT to bile and cationic antimicrobial peptides.

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References

SHORT PAPER: GENE DISRUPTION IN SALMONELLA TYPHIMURIM BY MODIFIED λ RED DISRUPTION SYSTEM

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APA: Copy

AHANI AZARI, ANIA, ZAHRAEI SALEHI, TAGHI, & NAYERI FASAEI, BAHAR. (2016). IN VITRO SUSCEPTIBILITY STUDY OF SALMONELLA TYPHIMURIUM MUTANT TO STRESS FACTORS. MEDICAL LABORATORY JOURNAL, 10(6), 28-31. SID. https://sid.ir/paper/684894/en

Vancouver: Copy

AHANI AZARI ANIA, ZAHRAEI SALEHI TAGHI, NAYERI FASAEI BAHAR. IN VITRO SUSCEPTIBILITY STUDY OF SALMONELLA TYPHIMURIUM MUTANT TO STRESS FACTORS. MEDICAL LABORATORY JOURNAL[Internet]. 2016;10(6):28-31. Available from: https://sid.ir/paper/684894/en

IEEE: Copy

ANIA AHANI AZARI, TAGHI ZAHRAEI SALEHI, and BAHAR NAYERI FASAEI, “IN VITRO SUSCEPTIBILITY STUDY OF SALMONELLA TYPHIMURIUM MUTANT TO STRESS FACTORS,” MEDICAL LABORATORY JOURNAL, vol. 10, no. 6, pp. 28–31, 2016, [Online]. Available: https://sid.ir/paper/684894/en

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