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Information Journal Paper

Title

EFFICACY OF HYALURONIC ACID IN THE SELECTION OF HUMAN SPERMATOZOA WITH INTACT DNA BY THE SWIM-UP METHOD

Pages

  83-88

Abstract

 Objective: In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of HYALURONIC ACID (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated.Materials and Methods: This observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. We divided each sample into two groups and the swim-up method was applied. HUMAN serum albumin (HSA, 0.5%) was added to samples from the first group. HA (10%) was added to samples from the second group. We determined the floating linear and non-linear sperm concentrations of both groups ANNEXIN V was used to determine the rate of APOPTOSIS of these sperm.Results: Following swim-up, linear and non-linear sperm concentrations were higher in the group that contained HA compared to the group with HSA. However, there was a significantly higher APOPTOSIS rate in the HSA group compared to the HA group.Conclusion: The addition of HA to the medium in the swim-up procedure positively affected sperm parameters. Thus, healthier sperm cells were obtained without DNA damage and with high motility.

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    APA: Copy

    SAYLAN, ASLIHAN, & DUMAN, SELCUK. (2016). EFFICACY OF HYALURONIC ACID IN THE SELECTION OF HUMAN SPERMATOZOA WITH INTACT DNA BY THE SWIM-UP METHOD. CELL JOURNAL (YAKHTEH), 18(1 (69)), 83-88. SID. https://sid.ir/paper/686163/en

    Vancouver: Copy

    SAYLAN ASLIHAN, DUMAN SELCUK. EFFICACY OF HYALURONIC ACID IN THE SELECTION OF HUMAN SPERMATOZOA WITH INTACT DNA BY THE SWIM-UP METHOD. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(1 (69)):83-88. Available from: https://sid.ir/paper/686163/en

    IEEE: Copy

    ASLIHAN SAYLAN, and SELCUK DUMAN, “EFFICACY OF HYALURONIC ACID IN THE SELECTION OF HUMAN SPERMATOZOA WITH INTACT DNA BY THE SWIM-UP METHOD,” CELL JOURNAL (YAKHTEH), vol. 18, no. 1 (69), pp. 83–88, 2016, [Online]. Available: https://sid.ir/paper/686163/en

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