Comparison of Detection of Clinical Isolates of Mycobacterium Tuberculosis by Flash PCR and Conventional Culture Method

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MORADABADI ALI REZA; ARJOMANDZADEGAN MOHAMMAD; Emami Navid; KAHBAZI MANIJEH; AHMADI AZAM; Falahat Saeed; HOSSEINI SEYYED HOSSEIN; KARGARAN MAHDI; KHOSRAVI PARISA

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2018 | Volume:21 | Issue:4 (133) Page(s): 77-85

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Title

Comparison of Detection of Clinical Isolates of Mycobacterium Tuberculosis by Flash PCR and Conventional Culture Method

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Keywords

TuberculosisQ1

Mycobacterium tuberculosisQ2

DetectionQ2

Flash PCRQ1

Culture methodQ1

Abstract

Background and Aim: Ziehl Nelson staining, fluorescent and also culture are the standard methods for the diagnosis of Tuberculosis. In this study, the performance of conventional cultivation methods was compared with Flash PCR. Materials and Methods: A total of 56 sputum samples from patients with suspected Tuberculosis in Tuberculosis Center of Arak city were collected and Ziehl– Neelsen and culture in Lö wenstein– Jensen medium were accomplished. Moreover, DNA from all of the 56 sputum samples was extracted by Chelex100 method. Molecular evaluation was accomplished by Flash PCR kit containing probes and primers for gene amplification IS6110. Positive and negative controls together with samples were used in a MTC410 apparatus for amplification. FD-12 apparatus was used to evaluate the results. In addition, electrophoresis on agarose was used for confirmation of the results. Findings: From 56 sputum samples of suspected TB patients, 20 samples were positive and 36 samples were negative on microscopic evaluation and Culture methods. FLASH-PCR molecular analysis showed that all of 20 positive samples were positive in molecular methods, too. On the other hand, three of sputum samples that were negative by culture and staining were positive in FLASH-PCR method. One of these 3 patients, received Isoniazid, pyrazinamide and ethambutol antibiotic by responsible medicine. All results were confirmed using conventional electrophoresis. Conclusion: In some negative samples, possibly because of the small number of bacteria in sample or a defect in the sampling, the Flash PCR may due good advantages. Therefore, due to the low cost, this method is recommended for routine use.

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APA: Copy

MORADABADI, ALI REZA, ARJOMANDZADEGAN, MOHAMMAD, Emami, Navid, KAHBAZI, MANIJEH, AHMADI, AZAM, Falahat, Saeed, HOSSEINI, SEYYED HOSSEIN, KARGARAN, MAHDI, & KHOSRAVI, PARISA. (2018). Comparison of Detection of Clinical Isolates of Mycobacterium Tuberculosis by Flash PCR and Conventional Culture Method. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 21(4 (133) ), 77-85. SID. https://sid.ir/paper/68865/en

Vancouver: Copy

MORADABADI ALI REZA, ARJOMANDZADEGAN MOHAMMAD, Emami Navid, KAHBAZI MANIJEH, AHMADI AZAM, Falahat Saeed, HOSSEINI SEYYED HOSSEIN, KARGARAN MAHDI, KHOSRAVI PARISA. Comparison of Detection of Clinical Isolates of Mycobacterium Tuberculosis by Flash PCR and Conventional Culture Method. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2018;21(4 (133) ):77-85. Available from: https://sid.ir/paper/68865/en

IEEE: Copy

ALI REZA MORADABADI, MOHAMMAD ARJOMANDZADEGAN, Navid Emami, MANIJEH KAHBAZI, AZAM AHMADI, Saeed Falahat, SEYYED HOSSEIN HOSSEINI, MAHDI KARGARAN, and PARISA KHOSRAVI, “Comparison of Detection of Clinical Isolates of Mycobacterium Tuberculosis by Flash PCR and Conventional Culture Method,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 21, no. 4 (133) , pp. 77–85, 2018, [Online]. Available: https://sid.ir/paper/68865/en

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