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Title

LOVASTATIN REDUCES STEMNESS VIA EPIGENETIC REPROGRAMING OF BMP2 AND GATA2 IN HUMAN ENDOMETRIUM AND ENDOMETRIOSIS

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Abstract

 Objective: The stem cell theory in the ENDOMETRIOSIS provides an advanced avenue of targeting these cells as a novel therapy to eliminate ENDOMETRIOSIS. In this regard, studies showed that LOVASTATIN alters the cells from a stem-like state to more differentiated condition and reduces STEMNESS. The aim of this study was to investigate whether LOVASTATIN treatment could influence expression and methylation patterns of genes regulating differentiation of endometrial mesenchymal stem cells (eMSCs) such asBMP2, GATA2 and RUNX2 as well as eMSCs markers.Materials and Methods: In this experimental investigation, MSCs were isolated from endometrial and endometriotic tissues and treated with LOVASTATIN and decitabin. To investigate the osteogenic and adipogenic differentiation of eMSCs treated with the different concentration of LOVASTATIN and decitabin, BMP2, RUNX2 and GATA2 expressions were measured by real-time polymerase chain reaction (PCR). To determine involvement of DNA methylation inBMP2 and GATA2 gene regulations of eMSCs, we used quantitative Methylation Specific PCR (qMSP) for evaluation of theBMP2 promoter status and differentially methylated region ofGATA2 exon 4.Results: In the present study, treatment with LOVASTATIN increased expression of BMP2 andRUNX2 and induced BMP2 promoter demethylation. We also demonstrated that LOVASTATIN treatment down-regulated GATA2 expression via inducing methylation. In addition, the results indicated that CD146 cell marker was decreased to 53% in response to LOVASTATIN treatment compared to untreated group.Conclusion: These findings indicated that LOVASTATIN treatment could increase the differentiation of eMSCs toward osteogenic and adiogenic lineages, while it decreased expression of eMSCs markers and subsequently reduced the STEMNESS.

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    APA: Copy

    TAGHIZADEH, MAHDIEH, & NORUZINIA, MEHRDAD. (2017). LOVASTATIN REDUCES STEMNESS VIA EPIGENETIC REPROGRAMING OF BMP2 AND GATA2 IN HUMAN ENDOMETRIUM AND ENDOMETRIOSIS. CELL JOURNAL (YAKHTEH), 19(1), 0-0. SID. https://sid.ir/paper/690556/en

    Vancouver: Copy

    TAGHIZADEH MAHDIEH, NORUZINIA MEHRDAD. LOVASTATIN REDUCES STEMNESS VIA EPIGENETIC REPROGRAMING OF BMP2 AND GATA2 IN HUMAN ENDOMETRIUM AND ENDOMETRIOSIS. CELL JOURNAL (YAKHTEH)[Internet]. 2017;19(1):0-0. Available from: https://sid.ir/paper/690556/en

    IEEE: Copy

    MAHDIEH TAGHIZADEH, and MEHRDAD NORUZINIA, “LOVASTATIN REDUCES STEMNESS VIA EPIGENETIC REPROGRAMING OF BMP2 AND GATA2 IN HUMAN ENDOMETRIUM AND ENDOMETRIOSIS,” CELL JOURNAL (YAKHTEH), vol. 19, no. 1, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/690556/en

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