MELISSA OFFICINALIS ACIDIC FRACTION PROTECTS CULTURED CEREBELLAR GRANULE NEURONS AGAINST BETA AMYLOID-INDUCED APOPTOSIS AND OXIDATIVE STRESS

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SOODI MALIHEH; DASHTI ABOLFAZL; HAJIMEHDIPOOR HOMA; AKBARI SHOLE; ATAEI NASIM

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Journal: Cell Journal (Yakhteh) Year:2017 | Volume:18 | Issue:4 Page(s): 556-564

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Title

MELISSA OFFICINALIS ACIDIC FRACTION PROTECTS CULTURED CEREBELLAR GRANULE NEURONS AGAINST BETA AMYLOID-INDUCED APOPTOSIS AND OXIDATIVE STRESS

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Keywords

MELISSA OFFICINALIS

NICOTINIC RECEPTOR

APOPTOSIS

ALZHEIMER’S DISEASE

Abstract

Objective: Extracellular deposition of the beta-amyloid (Ab) peptide, which is the main finding in the pathophysiology of ALZHEIMER’S DISEASE (AD), leads to oxidative damage and APOPTOSIS in neurons. MELISSA OFFICINALIS (M. officinalis) is a medicinal plant from the Lamiaceae family that has neuroprotective activity. In the present study we have investigated the protective effect of the acidic fraction of M. officinalis on Ab-induced oxidative stress and APOPTOSIS in cultured cerebellar granule neurons (CGN). Additionally, we investigated a possible role of the NICOTINIC RECEPTOR.Materials and Methods: This study was an in vitro experimental study performed on mice cultured CGNs. CGNs were pre-incubated with different concentrations of the acidic fraction of M. officinalis for 24 hours, followed by incubation with Ab for an additional 48 hours. CGNs were also pre-incubated with the acidic fraction of M. officinalis and mecamylamin, followed by incubation with Ab. We used the 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay to measure cell viability. Acetylcholinesterase (AChE) activity, reactive oxygen species (ROS) production, lipidperoxidation, and caspase-3 activity were measured after incubation. Hochst/annexin V- fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining was performed to detect apoptotic cells.Results: The acidic fraction could protect CGNs from Ab-induced cytotoxicity. Mecamylamine did not abolish the protective effect of the acidic fraction. AChE activity, ROS production, lipid peroxidation, and caspase-3 activity increased after Ab incubation. Preincubation with the acidic fraction of M. officinalis ameliorated these factors and decreased the number of apoptotic cells.Conclusion: Our results indicated that the protective effect of the acidic fraction of M. officinalis was not mediated through NICOTINIC RECEPTORs. This fraction could protect CGNs through antioxidant and anti-apoptotic activities.

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APA: Copy

SOODI, MALIHEH, DASHTI, ABOLFAZL, HAJIMEHDIPOOR, HOMA, AKBARI, SHOLE, & ATAEI, NASIM. (2017). MELISSA OFFICINALIS ACIDIC FRACTION PROTECTS CULTURED CEREBELLAR GRANULE NEURONS AGAINST BETA AMYLOID-INDUCED APOPTOSIS AND OXIDATIVE STRESS. CELL JOURNAL (YAKHTEH), 18(4), 556-564. SID. https://sid.ir/paper/698331/en

Vancouver: Copy

SOODI MALIHEH, DASHTI ABOLFAZL, HAJIMEHDIPOOR HOMA, AKBARI SHOLE, ATAEI NASIM. MELISSA OFFICINALIS ACIDIC FRACTION PROTECTS CULTURED CEREBELLAR GRANULE NEURONS AGAINST BETA AMYLOID-INDUCED APOPTOSIS AND OXIDATIVE STRESS. CELL JOURNAL (YAKHTEH)[Internet]. 2017;18(4):556-564. Available from: https://sid.ir/paper/698331/en

IEEE: Copy

MALIHEH SOODI, ABOLFAZL DASHTI, HOMA HAJIMEHDIPOOR, SHOLE AKBARI, and NASIM ATAEI, “MELISSA OFFICINALIS ACIDIC FRACTION PROTECTS CULTURED CEREBELLAR GRANULE NEURONS AGAINST BETA AMYLOID-INDUCED APOPTOSIS AND OXIDATIVE STRESS,” CELL JOURNAL (YAKHTEH), vol. 18, no. 4, pp. 556–564, 2017, [Online]. Available: https://sid.ir/paper/698331/en

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