EFFECT OF SALVIA CHORASSANICA ROOT AQUEOUS, ETHANOLIC AND HYDRO ALCOHOLIC EXTRACTS ON STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI

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MEHRABAN AZAM

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Journal: Zahedan Journal of Research in Medical Sciences Year:2016 | Volume:18 | Issue:11 Page(s): 0-0

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Title

EFFECT OF SALVIA CHORASSANICA ROOT AQUEOUS, ETHANOLIC AND HYDRO ALCOHOLIC EXTRACTS ON STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI

Author(s)

MEHRABAN AZAM | Issue Writer Certificate

Keywords

ANTIBACTERIAL AGENT

DILUTION TECHNIQUES

DISK DIFFUSION ANTIMICROBIAL TESTS

SALVIA

Abstract

Background: Nowadays, through the previous researches, it has become clear that SALVIA has important health benefits. SALVIA chorassanicais one of the valuable native Iranian species which only grows in Khorasan province, Iran.Objectives: The aim of this study is to evaluate the antimicrobial effect of SALVIA chorassanica root aqueous, ethanolic and hydro alcoholic extracts onStaphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium and Escherichia coli.Methods: In this experimental study, maceration method was used to prepare extracts. Study setup was conducted in March 2014.The duration of study setup took for two months. The micro dilution method by ELISA was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of aqueous, ethanolic and hydro alcoholic extracts of root ofSalvia chorassanica against Staphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium and Escherichia coli. The antibacterial effect also was evaluated using agar diffusion method. The inhibition zones of growth against the extracts were measured in comparison to standards antibiotics. Chloramphenicol as positive control onEnterococcus faecalis, Tetracycline on Staphylococcus aureus, Gentamicin on Escherichia coli and Neomycin on Salmonella typhimurium. The data were analyzed using one way analysis of variance (ANOVA) with SPSS version 16.Results: The highest inhibition zone in diffusionmethod was related to ethanolic extract of SALVIA chorassanica root against Grampositive bacteria, Staphylococcus aureus and Enterococcus faecalis. The calculated MIC in aqueous and ethanolic extracts of root for Staphylococcus aureuswas 240 and 120 mg/mL, for Enterococcus faecalis was 120 and 60mg/mLrespectively, and for Escherichia coli and Salmonella typhimuriumwas equal to 240 mg/mL. The amount in hydro alcoholic extracts for Gram-positive bacteria was 60 mg/mL and for Gram-negative bacteria was 120 mg/mL. The extract ofSalvia chorassanica root was showed acceptable antimicrobial effect against studied bacteria.Conclusions: The results of the disk agar diffusion and micro dilution showed the highest inhibitory effect on the tested bacteria was related to ethanolic and hydro alcoholic extracts of rootSalvia chorassanica. It was also observed that the Gram-positive bacteria compared to the Gram- negative bacteria, have greater sensitivity against different extracts ofSalvia chorassanica.

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APA: Copy

MEHRABAN, AZAM. (2016). EFFECT OF SALVIA CHORASSANICA ROOT AQUEOUS, ETHANOLIC AND HYDRO ALCOHOLIC EXTRACTS ON STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 18(11), 0-0. SID. https://sid.ir/paper/700299/en

Vancouver: Copy

MEHRABAN AZAM. EFFECT OF SALVIA CHORASSANICA ROOT AQUEOUS, ETHANOLIC AND HYDRO ALCOHOLIC EXTRACTS ON STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2016;18(11):0-0. Available from: https://sid.ir/paper/700299/en

IEEE: Copy

AZAM MEHRABAN, “EFFECT OF SALVIA CHORASSANICA ROOT AQUEOUS, ETHANOLIC AND HYDRO ALCOHOLIC EXTRACTS ON STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 18, no. 11, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/700299/en

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