THE IN VITRO STUDY OF INHIBITORY PROPERTIES OF TRITERPENES FROMEXTRACTED EUPHORBIA PLANT ON CYCLOOXYGENASE-1 ACTIVITY AND THE EVALUATION OF THEIR CYTOTOXICITY ON L-929 CELL LINES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

PALIZBAN A.A.; GHANADIAN S.M.; SADEGHI H.; GHAISARI B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES Year:2015 | Volume:14 | Issue:8 Page(s): 665-678

Download Full-Text

Persian Verion

View:

1,891

Download:

Cites:

Information Journal Paper

Title

THE IN VITRO STUDY OF INHIBITORY PROPERTIES OF TRITERPENES FROMEXTRACTED EUPHORBIA PLANT ON CYCLOOXYGENASE-1 ACTIVITY AND THE EVALUATION OF THEIR CYTOTOXICITY ON L-929 CELL LINES

Author(s)

PALIZBAN A.A. | GHANADIAN S.M. | SADEGHI H. | GHAISARI B. | Issue Writer Certificate

Keywords

TRITERPENESQ2

CYCLOOXYGENASE-1Q3

CYCLOARTANQ1

INFLAMMATORY DISEASESQ2

L-929 CELL LINESQ2

Abstract

Background and Objective: TRITERPENES could exert anti-inflammatory properties. The aim of this study was to evaluate the inhibitory activities of CYCLOARTAN triterpene derivatives extracted from Euphorbia with names as; CYCLOARTAN-24-methylene3-ol (1), CYCLOARTAN-23-ene-3, 25-diol (2), CYCLOARTAN-23-ene-25-methoxy-3-ol (3) and CYCLOARTAN-24-ene-3-ol (4) on CYCLOOXYGENASE-1 (cox-1) activity.Materials and Methods: This is an experimental study. The cyclooxygenase enzyme was extracted from platelets and peripheral blood mononuclear cells by Ficoll-Paque density gradient. The inhibitory activities of the compounds were assessed by measuring the peroxidase activity of enzyme by End point Enzyme activity assay.The inhibitory effects of the compounds were examined using logarithmic concentrations of them (1, 10, 100 mg/ml). The cytotoxicity of each compound was measured using Cell Survival of L-929 CELL LINES by MTT assay.Results: The cox-1 inhibitory effect of compound 3 was very weak at a concentration more than 100 mg/ml and IC50>100 (mg/ml). The results revealed that; the compounds 1, 2 and 4 at concentrations (1, 10, and 100 μg/ml) demonstrated the acceptable inhibitory effects for the cox-1 enzyme with the IC50 of 9.65, 16.51 and 9.22 (mg/ml) compared with SC-560 control, respectively. The toxicity effects of compounds compared with Doxorubicin on the survival of L-929 cells, revealed that the IC50 of the 1, 2, 3, and 4 compounds are equal to 64.88, 64.47, >60 and 66.23 (mg/ml), respectively.Conclusion: CYCLOARTAN triterpene derivatives extracted from Euphorbia plant could have inhibitory activities on cox-1 enzyme. The cytotoxicity study showed that these compounds probably at low concentrations could be useful to treat the diseases caused by increasing cycloxygenese-1 activity.

Cites

No record.

References

No record.

Cite

APA: Copy

PALIZBAN, A.A., GHANADIAN, S.M., SADEGHI, H., & GHAISARI, B.. (2015). THE IN VITRO STUDY OF INHIBITORY PROPERTIES OF TRITERPENES FROMEXTRACTED EUPHORBIA PLANT ON CYCLOOXYGENASE-1 ACTIVITY AND THE EVALUATION OF THEIR CYTOTOXICITY ON L-929 CELL LINES. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 14(8), 665-678. SID. https://sid.ir/paper/70813/en

Vancouver: Copy

PALIZBAN A.A., GHANADIAN S.M., SADEGHI H., GHAISARI B.. THE IN VITRO STUDY OF INHIBITORY PROPERTIES OF TRITERPENES FROMEXTRACTED EUPHORBIA PLANT ON CYCLOOXYGENASE-1 ACTIVITY AND THE EVALUATION OF THEIR CYTOTOXICITY ON L-929 CELL LINES. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2015;14(8):665-678. Available from: https://sid.ir/paper/70813/en

IEEE: Copy

A.A. PALIZBAN, S.M. GHANADIAN, H. SADEGHI, and B. GHAISARI, “THE IN VITRO STUDY OF INHIBITORY PROPERTIES OF TRITERPENES FROMEXTRACTED EUPHORBIA PLANT ON CYCLOOXYGENASE-1 ACTIVITY AND THE EVALUATION OF THEIR CYTOTOXICITY ON L-929 CELL LINES,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 14, no. 8, pp. 665–678, 2015, [Online]. Available: https://sid.ir/paper/70813/en

Related Journal Papers