EVALUATION OF TOTAL PHENOLIC, FLAVONOID, ANTHOCYANIN COMPOUNDS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF HAWTHORN (CRATAEGUS ELBURSENSIS) FRUIT ACETONIC EXTRACT

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SALMANIAN SH.; SADEGHI MAHOUNAK A.R.; ALAMI M.; GHORBANI M.

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Paper Information

Journal: Journal of Rafsanjan University of Medical Sciences Year:2014 | Volume:13 | Issue:1 Page(s): 53-66

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Title

EVALUATION OF TOTAL PHENOLIC, FLAVONOID, ANTHOCYANIN COMPOUNDS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF HAWTHORN (CRATAEGUS ELBURSENSIS) FRUIT ACETONIC EXTRACT

Author(s)

SALMANIAN SH. | SADEGHI MAHOUNAK A.R. | ALAMI M. | GHORBANI M. | Issue Writer Certificate

Keywords

ANTIMICROBIAL ACTIVITYQ1

TOTAL PHENOLIC COMPOUNDSQ2

ANTIOXIDANTQ2

HAWTHORN FRUITQ2

Abstract

Background and Objective: Crataegus elbursensi s is one of the important species of Rosaceae family and commonly found in the North of Iran. In this study, the ANTIOXIDANT, antibacterial properties, total phenolic, flavonoid and anthocyanin contents in acetonic extract of Iranian Hawthorn (Crataegus elbursensis) fruits were examined.Materials and Methods: Extraction of fruit bioactive compound was done using %80 acetone. Total phenolic, flavonoid and anthocyanin contents were measured spectrophotometrically. The ANTIOXIDANT capacity of the extract were assessed by DPPH (2, 2-diphenyl-1- picrylhydrazyl) radical-scavenging activity and compared to synthetic ANTIOXIDANT BHT (at different concentration of 50, 75, 100, 250, 500 mg/ml). In addition, the stability of the acetonic extract to pH (4, 7 and 9) and also ANTIMICROBIAL ACTIVITY of the extract by the broth microdilution method was investigated. Data were analyzed using Duncan's multiple test by SAS software Results: The extract showed higher radical scavenging activity than BHT. Also, the results of extract stability tests in different pH showed that ANTIOXIDANT activity of the extract remained constant. The extract revealed significant ANTIMICROBIAL ACTIVITY against all of tested bacterial. The most efficient bactericidal activity of the extract was against Bacillus subtilis (MBC=2.5 mg/ml) while Salmonella enteric was the most resistant bacterium (MBC=20 mg/ml).Conclusion: The results obtained in this study clearly indicate that C. elbursensis studied proved to be a rich source of bioactive compounds with ANTIOXIDANT and antimicrobial properties which could be used in food and drug industry and serve to protect human health.

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APA: Copy

SALMANIAN, SH., SADEGHI MAHOUNAK, A.R., ALAMI, M., & GHORBANI, M.. (2014). EVALUATION OF TOTAL PHENOLIC, FLAVONOID, ANTHOCYANIN COMPOUNDS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF HAWTHORN (CRATAEGUS ELBURSENSIS) FRUIT ACETONIC EXTRACT. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 13(1), 53-66. SID. https://sid.ir/paper/71044/en

Vancouver: Copy

SALMANIAN SH., SADEGHI MAHOUNAK A.R., ALAMI M., GHORBANI M.. EVALUATION OF TOTAL PHENOLIC, FLAVONOID, ANTHOCYANIN COMPOUNDS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF HAWTHORN (CRATAEGUS ELBURSENSIS) FRUIT ACETONIC EXTRACT. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2014;13(1):53-66. Available from: https://sid.ir/paper/71044/en

IEEE: Copy

SH. SALMANIAN, A.R. SADEGHI MAHOUNAK, M. ALAMI, and M. GHORBANI, “EVALUATION OF TOTAL PHENOLIC, FLAVONOID, ANTHOCYANIN COMPOUNDS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF HAWTHORN (CRATAEGUS ELBURSENSIS) FRUIT ACETONIC EXTRACT,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 13, no. 1, pp. 53–66, 2014, [Online]. Available: https://sid.ir/paper/71044/en

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