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Information Journal Paper

Title

PolyI: C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling

Pages

  325-333

Abstract

 Objective: This study aimed to evaluate the specific roles of polyinosinic: polycytidylic acid (polyI: C) in macrophage Chemotaxis and reveal the potential regulatory mechanisms related to Chemokine Receptor 5 (CCR5). Materials and Methods: In this experimental study, THP-1-derived Macrophages (THP1-Mφ s) induced from THP-1 monocytes were treated with 25 μ g/mL polyI: C. Toll-like receptor 3 (TLR3), Jumonji domain-containing protein (JMJD)1A, and JMJD1C small interfering RNA (siRNAs) were transfected into THP1-Mφ s. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used to detect the expression levels of TLR3, CCR5, 23 Jumonji C domain-containing histone demethylase family members, JMJD1A, and JMJD1C in THP1-Mφ s with different siRNAs transfections. Western blot was performed to detect JMJD1A, JMJD1C, H3K9me2, and H3K9me3 expressions. A transwell migration assay was conducted to detect THP1-Mφ Chemotaxis toward chemokine ligand 3 (CCL3). A chromatin immunoprecipitation (ChIP) assay was performed to detect H3K9me2-CCR5 complexes in THP1-Mφ s. Results: PolyI: C significantly upregulated CCR5 in THP1-Mφ s and promoted Chemotaxis toward CCL3 (P<0. 05); these effects were significantly inhibited by TLR3 siRNA (P<0. 01). JMJD1A and JMJD1C expression was significantly upregulated in polyI: C-stimulated THP1-Mφ s, while only JMJD1A siRNA decreased CCR5 expression (P<0. 05). JMJD1A siRNA significantly increased H3K9me2 expression in THP1-Mφ s but not in polyI: C-stimulated THP1-Mφ s. The ChIP result revealed that polyI: C significantly downregulated H3K9me2 in the promoter region of CCR5 in THP1-Mφ s. Conclusion: PolyI: C can enhance THP1-Mφ Chemotaxis toward CCL3 regulated by TLR3/JMJD1A signalling and activate CCR5 expression by reducing H3K9me2 in the promoter region of CCR5.

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    APA: Copy

    Xiaoxiao, Yu, Wang, Huayang, Shao, Hongjia, Zhang, Cuijuan, Ju, Xiuli, & YANG, JIE. (2020). PolyI: C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling. CELL JOURNAL (YAKHTEH), 22(3), 325-333. SID. https://sid.ir/paper/728128/en

    Vancouver: Copy

    Xiaoxiao Yu, Wang Huayang, Shao Hongjia, Zhang Cuijuan, Ju Xiuli, YANG JIE. PolyI: C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(3):325-333. Available from: https://sid.ir/paper/728128/en

    IEEE: Copy

    Yu Xiaoxiao, Huayang Wang, Hongjia Shao, Cuijuan Zhang, Xiuli Ju, and JIE YANG, “PolyI: C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling,” CELL JOURNAL (YAKHTEH), vol. 22, no. 3, pp. 325–333, 2020, [Online]. Available: https://sid.ir/paper/728128/en

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