PURIFICATION OF IMMUNOGLOBULIN Y (IGY) FROM EGG YOLK BY GEL FILTRATION CHROMATOGRAPHY

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POURAMIR M.; RASAEI M.J.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2001 | Volume:3 | Issue:2 (10) Page(s): 7-11

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Information Journal Paper

Title

PURIFICATION OF IMMUNOGLOBULIN Y (IGY) FROM EGG YOLK BY GEL FILTRATION CHROMATOGRAPHY

Author(s)

POURAMIR M. | RASAEI M.J. | Issue Writer Certificate

Keywords

IMMUNOGLOBULIN Y(IGY)Q4

GEL FILTRATIONQ3

PURIFICATIONQ3

Abstract

OBJECTIVE: PURIFICATION of immunoglobulin Y from egg yolk by chromatography is a new investigation. In this study, we developed an easy and inexpensive method for PURIFICATION of IgY from egg yolk. METHODS: The yolk from egg was separated and passed through a nylon mesh into a measuring cylinder. The egg yolk was diluted 10 times with HCL (3 mM) and prepared suspension was centrifuged. Solid ammonium sulfate was added to 60% saturation. The precipitate was dissolved in phosphate buffer and applied on the gel-filtration columns (sephadex G25, G200). FINDINGS: The result of cellulose acetate electrophoresis at pH=9 showed some extra bands which were isolated with ammonium sulfate precipitation and sephadex G-25 chromatography. The purified band was observed in the y-globulin region. After sephadex G-200 chromatography, SDS-PAGE electrophoresis of the product showed one band about 63 KD. CONCLUSION: IgY extraction and PURIFICATION with acid, centrifugation, ammonium sulfate and GEL FILTRATION are effective and simple operative methods.

Cites

IMMUNOGLOBULIN Y (IGY) FROM EGG YOLK: FROM MOLECULAR STRUCTURES TO MEDICAL APPLICATIONS

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APA: Copy

POURAMIR, M., & RASAEI, M.J.. (2001). PURIFICATION OF IMMUNOGLOBULIN Y (IGY) FROM EGG YOLK BY GEL FILTRATION CHROMATOGRAPHY. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 3(2 (10)), 7-11. SID. https://sid.ir/paper/73744/en

Vancouver: Copy

POURAMIR M., RASAEI M.J.. PURIFICATION OF IMMUNOGLOBULIN Y (IGY) FROM EGG YOLK BY GEL FILTRATION CHROMATOGRAPHY. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2001;3(2 (10)):7-11. Available from: https://sid.ir/paper/73744/en

IEEE: Copy

M. POURAMIR, and M.J. RASAEI, “PURIFICATION OF IMMUNOGLOBULIN Y (IGY) FROM EGG YOLK BY GEL FILTRATION CHROMATOGRAPHY,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 3, no. 2 (10), pp. 7–11, 2001, [Online]. Available: https://sid.ir/paper/73744/en

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