Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

259
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

152
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Short Pretreatment with Calcitriol Is Far Superior to Continuous Treatment in Stimulating Proliferation and Osteogenic Differentiation of Human Adipose Stem Cells

Pages

  293-301

Abstract

 Objective: This study investigated whether short stimulation (30 minutes) of human adipose stem cells (hASCs) with 1, 25-dihydroxyvitamin D3 (calcitriol or 1, 25-(OH)2VitD3), fitting within the surgical procedure time frame, suffices to induce osteogenic differentiation, and compared this with continuous treatment with 1, 25-(OH)2VitD3. Materials and Methods: In this experimental study, hASCs were pretreated with/without 10 nM calcitriol for 30 minutes, seeded on biphasic calcium phosphate (BCP), and cultured for 3 weeks with/without 1, 25-(OH)2VitD3. Cell attachment was determined 30 minutes after cell seeding. AlamarBlue assay, alkaline phosphatase (ALP) assay, ALP staining, real-time polymerase chain reaction (PCR), and protein assay were used to evaluate the effect of short calcitriol pretreatment on Proliferation and osteogenic differentiation of hASCs up to 3 weeks. Results: Pretreatment with 1, 25-(OH)2VitD3 enhanced the attachment of hASCs to BCP by 1. 5-fold compared to nontreated cells and increased the Proliferation by 3. 5-fold at day 14, and 2. 6-fold at day 21. In contrast, continuous treatment increased the Proliferation by 1. 7-fold only at day 14. After 2 weeks, ALP activity was increased by 18. 5-fold when hASCs were pretreated with 1, 25-(OH)2VitD3 for 30 minutes but increased only 2. 6-fold when compared with its continuous counterpart. Moreover, after 14 days, pretreatment resulted in significant upregulation of the osteogenic markers RUNX2 and SPARC by 3. 6-fold and 2. 2-fold, respectively, while this was not observed upon continuous treatment. Finally, 30 minutes pretreatment of hASCs with 1, 25-(OH)2VitD3 increased VEGF189 expression, which may contribute to the process of angiogenesis. Conclusion: This study is the first research showing that 30 minutes pretreatment of hASCs with 1, 25-(OH)2VitD3, not only enhanced cell attachment to the scaffold at seeding time, but also promoted the Proliferation and osteogenic differentiation of hASCs more strongly than continuous treatment, suggesting that short pre-treatment with 1, 25-(OH)2VitD3 is a promising approach for the regeneration of Bones in a one-step surgical procedure.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Mokhtari Jafari, Fatemeh, DEHGHAN, MOHAMMAD MEHDI, HELDER, MARCO N., ZANDIEH DOULABI, BEHROUZ, Klein Nulend, Jenneke, & AMOABEDINY, GHASSEM. (2020). Short Pretreatment with Calcitriol Is Far Superior to Continuous Treatment in Stimulating Proliferation and Osteogenic Differentiation of Human Adipose Stem Cells. CELL JOURNAL (YAKHTEH), 22(3), 293-301. SID. https://sid.ir/paper/738546/en

    Vancouver: Copy

    Mokhtari Jafari Fatemeh, DEHGHAN MOHAMMAD MEHDI, HELDER MARCO N., ZANDIEH DOULABI BEHROUZ, Klein Nulend Jenneke, AMOABEDINY GHASSEM. Short Pretreatment with Calcitriol Is Far Superior to Continuous Treatment in Stimulating Proliferation and Osteogenic Differentiation of Human Adipose Stem Cells. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(3):293-301. Available from: https://sid.ir/paper/738546/en

    IEEE: Copy

    Fatemeh Mokhtari Jafari, MOHAMMAD MEHDI DEHGHAN, MARCO N. HELDER, BEHROUZ ZANDIEH DOULABI, Jenneke Klein Nulend, and GHASSEM AMOABEDINY, “Short Pretreatment with Calcitriol Is Far Superior to Continuous Treatment in Stimulating Proliferation and Osteogenic Differentiation of Human Adipose Stem Cells,” CELL JOURNAL (YAKHTEH), vol. 22, no. 3, pp. 293–301, 2020, [Online]. Available: https://sid.ir/paper/738546/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top