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Information Journal Paper

Title

STUDY ON MECHANISM OF VASORELAXATORY EFFECT OF VITIS VINIFERA LEAF EXTRACT IN RAT AORTA

Pages

  71-77

Keywords

RATQ3
K+ CHANNELSQ3

Abstract

 Introduction: Our previous studies showed that hydroalcoholic extract of leaf of VITIS VINIFERA relaxes the phenylephrine-induced contraction in RAT thoracic AORTA. This effect was dependent on endothelial integrity and NO-cGMP system. The vasorelaxant effect of extract was much lesser on KCl-induced contraction. We, therefore, postulated that K+ channels are involved. The main aim of the present study was to determine the type of K+ channels involved in this vasorelaxant effect.Methods: Thoracic AORTA with intact endothelium was removed from adult male Wistar RATs (170-220g). The AORTA was mounted in an organ bath containing Krebs-Henseleit (37 °C, pH 7.4) bubbled with O2. Aortic contractions were recorded isometrically under 1 g resting tension. The AORTA endothelium was considered intact if acetylcholine (1 mM) could induce more than 70% AORTA relaxation on 1μM phenylephrine-induced contraction. Extract was prepared by maceration method using 70% alcohol and the solvent was then evaporated.Results: The results showed that in the presence of tetraethyl ammonium (10 mM), the vasorelaxant effect of extract (0.25, 0.5, 1 and 2 mg/ml) was reduced (P<0.001, n=7). In contrast, glibenclamide (1 mM) had no effect. In calcium-free (plus 0.1 mM of EDTA) Krebs-Henseleit solution, the vasorelaxant effect of extract (0.25, 0.5 and 1 mg/ml) was reduced (P<0.0001, n=8). Furthermore, the vasorelaxant effect of extract was unaffected by indomethacin (1 mM).Conclusion: These results suggest that VITIS VINIFERA leaf hydroalcoholic extract induces relaxation in RAT AORTA possibly by opening the Ca2+ -operated K+ channels but, not ATP- sensitive K+ channels and extracellular calcium was essential for inducing vasorelaxation by extract. Furthermore, cyclooxigenase was not involved in this vasorelaxant effect.

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    APA: Copy

    GHARIB NASERI, MOHAMMAD KAZEM, & HEYDARI, AKBAR. (2006). STUDY ON MECHANISM OF VASORELAXATORY EFFECT OF VITIS VINIFERA LEAF EXTRACT IN RAT AORTA. PHYSIOLOGY AND PHARMACOLOGY, 10(1), 71-77. SID. https://sid.ir/paper/74922/en

    Vancouver: Copy

    GHARIB NASERI MOHAMMAD KAZEM, HEYDARI AKBAR. STUDY ON MECHANISM OF VASORELAXATORY EFFECT OF VITIS VINIFERA LEAF EXTRACT IN RAT AORTA. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2006;10(1):71-77. Available from: https://sid.ir/paper/74922/en

    IEEE: Copy

    MOHAMMAD KAZEM GHARIB NASERI, and AKBAR HEYDARI, “STUDY ON MECHANISM OF VASORELAXATORY EFFECT OF VITIS VINIFERA LEAF EXTRACT IN RAT AORTA,” PHYSIOLOGY AND PHARMACOLOGY, vol. 10, no. 1, pp. 71–77, 2006, [Online]. Available: https://sid.ir/paper/74922/en

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