مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

803
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DEHYDROEPIANDROSTERONE INCREASES THE PROLIFERATION OF NEURAL PROGENITOR CELLS DERIVED FROM P19 EMBRYONAL CARCINOMA STEM CELLS

Pages

  180-187

Abstract

 Introduction: The p19 line of embryonal carcinoma cells develop into NEURONs, astroglia and fibroblasts after aggregation and exposure to retinoic acid (RA). Dehydroepiandrosterone (DHEA) is a neurosteroid, which can increase the proliferation of human neural stem cells (NSC) and positively regulate the number of NEURONs produced. This study was initiated to assess the effect of DHEA on neural progenitor cells derived from P19 EMBRYONAL CARCINOMA STEM CELLS. Methods: p19 cells were suspended in DMEM containing 5% FBS in bacterial-grade Petri dishes in the presence of RA and DHEA at different concentrations for 6 days. Serum concentration was decreased to 3% on days 5 and 6. The aggregates were then collected and processed for flow cytometry, immunocytochemistry and RT-PCR analyses. Cells were trypsinized for dispersion and were placed in to poly-L-lysine (10 mg/ml) coated tissue culture dishes without RA and DHEA for 4 days. Differentiated cells were then evaluated by phase contrast microscopy.Results: Flow cytometry analyses of Nestin and Brdu/Nestin showed percent Nestin positive and proliferating Nestin positive cells in different groups including DHEA and RA groups. Brdul/Nestin immunochemistry confirmed proliferation of Nestin positive cells and also RT-PCR analysis showed the expression of proneural markers and estrogen receptor genes. Result showed that RA + DHEA (mM) significantly increased the number of Nestin positive and newly formed Nestin positive cells compared to other groups.Conclusion: These results showed that DHEA accompanied by RA significantly increased the number of Nestin positive and newly formed Nestin positive cells derived from p19 embryonal carcinoma cells but in comparison to RA could not induce neural progenitor cells.

Cites

  • No record.
  • References

  • No record.
  • Cite

    APA: Copy

    AZIZI, HOSSEIN, ZARE MEHRJERDI, N., KAZEMI ASHTIANI, S., BAHMANI, M.KH., & BAHARVAND, HOSSEIN. (2008). DEHYDROEPIANDROSTERONE INCREASES THE PROLIFERATION OF NEURAL PROGENITOR CELLS DERIVED FROM P19 EMBRYONAL CARCINOMA STEM CELLS. PHYSIOLOGY AND PHARMACOLOGY, 12(3), 180-187. SID. https://sid.ir/paper/75243/en

    Vancouver: Copy

    AZIZI HOSSEIN, ZARE MEHRJERDI N., KAZEMI ASHTIANI S., BAHMANI M.KH., BAHARVAND HOSSEIN. DEHYDROEPIANDROSTERONE INCREASES THE PROLIFERATION OF NEURAL PROGENITOR CELLS DERIVED FROM P19 EMBRYONAL CARCINOMA STEM CELLS. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2008;12(3):180-187. Available from: https://sid.ir/paper/75243/en

    IEEE: Copy

    HOSSEIN AZIZI, N. ZARE MEHRJERDI, S. KAZEMI ASHTIANI, M.KH. BAHMANI, and HOSSEIN BAHARVAND, “DEHYDROEPIANDROSTERONE INCREASES THE PROLIFERATION OF NEURAL PROGENITOR CELLS DERIVED FROM P19 EMBRYONAL CARCINOMA STEM CELLS,” PHYSIOLOGY AND PHARMACOLOGY, vol. 12, no. 3, pp. 180–187, 2008, [Online]. Available: https://sid.ir/paper/75243/en

    Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    مرکز اطلاعات علمی SID
    strs
    دانشگاه امام حسین
    بنیاد ملی بازیهای رایانه ای
    کلید پژوه
    ایران سرچ
    ایران سرچ
    File Not Exists.
    Move to top