EVALUATION OF THE EFFECT OF CHICK EMBRYONIC NOTOCHORD ON NEURAL INDUCTION AND DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS

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ANJOMSHOA M.; KARBALAEI KH.; RAZAVI SHAHNAZ; MARDANI M.; TANHAEI S.; NASRESFAHANI MOHAMMAD HOSSEIN; BAHARVAND HOSSEIN

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Journal: Physiology and Pharmacology Year:2008 | Volume:12 | Issue:1 Page(s): 1-13

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Title

EVALUATION OF THE EFFECT OF CHICK EMBRYONIC NOTOCHORD ON NEURAL INDUCTION AND DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS

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Keywords

NOTOCHORDQ3

NEURAL INDUCTIONQ3

EMBRYONIC STEM CELLSQ3

CO-CULTUREQ3

RETINOIC ACIDQ3

Abstract

Introduction: The aim of this study was to evaluate the ability of the NOTOCHORD to induce differentiation of mouse EMBRYONIC STEM CELLS to neurons and/or motor neurons.Methods: In order to produce embryoid bodies (EBs), mouse EMBRYONIC STEM CELLS (ES cells) line Royan Bl were grown in suspension in the absence of LIF for 4 days. Then EBs were divided into 4 groups. EBs in groups 1 and 2 were further cultured in suspension for 4 days in the presence of RETINOIC ACID (RA) while EBs in groups 3 and 4 were cultured in the absence of RA. EBs in groups 1 and 3 were also CO-CULTUREd with NOTOCHORD for 4 more days. Numbers and type of neurons were assessed by immunostaining, flow cytometry and RT-PCR techniques.Results: EBs in groups 3 and 4 lead to 5 to 6 % neuron production while EBs in the groups 1 and 2 lead to 42 and 55% neuron production, respectively. There was a significant difference (P <0.05) between groups 3 and 4 with groups 1 and 2. Percentage of motor neurons (Hb9 positive) were significantly higher in group 1 (20.4%) compared with other groups (2-3%).Conclusion: CO-CULTURE of mouse EMBRYONIC STEM CELLS in the presence of NOTOCHORD did not induce neural differentiation of mouse EMBRYONIC STEM CELLS, while NOTOCHORD may direct neural differentiation of mouse EMBRYONIC STEM CELLS toward motor neurons.

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APA: Copy

ANJOMSHOA, M., KARBALAEI, KH., RAZAVI, SHAHNAZ, MARDANI, M., TANHAEI, S., NASRESFAHANI, MOHAMMAD HOSSEIN, & BAHARVAND, HOSSEIN. (2008). EVALUATION OF THE EFFECT OF CHICK EMBRYONIC NOTOCHORD ON NEURAL INDUCTION AND DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS. PHYSIOLOGY AND PHARMACOLOGY, 12(1), 1-13. SID. https://sid.ir/paper/75258/en

Vancouver: Copy

ANJOMSHOA M., KARBALAEI KH., RAZAVI SHAHNAZ, MARDANI M., TANHAEI S., NASRESFAHANI MOHAMMAD HOSSEIN, BAHARVAND HOSSEIN. EVALUATION OF THE EFFECT OF CHICK EMBRYONIC NOTOCHORD ON NEURAL INDUCTION AND DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2008;12(1):1-13. Available from: https://sid.ir/paper/75258/en

IEEE: Copy

M. ANJOMSHOA, KH. KARBALAEI, SHAHNAZ RAZAVI, M. MARDANI, S. TANHAEI, MOHAMMAD HOSSEIN NASRESFAHANI, and HOSSEIN BAHARVAND, “EVALUATION OF THE EFFECT OF CHICK EMBRYONIC NOTOCHORD ON NEURAL INDUCTION AND DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS,” PHYSIOLOGY AND PHARMACOLOGY, vol. 12, no. 1, pp. 1–13, 2008, [Online]. Available: https://sid.ir/paper/75258/en

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