Characterizing and controlling the loading of Vipera albicornuta venom in chitosan nanoparticles as an adjuvant and vaccine delivery system

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Shambayati Mohammad Hassan; MEHRABI MOHSEN; Mohammadpour Dounighi Nasser; RAMAZANI ALI; ZARE MIRAKABADI ABBAS; AHMADI EBRAHIM

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Paper Information

Journal: NANOMEDICINE RESEARCH JOURNAL Year:2019 | Volume:4 | Issue:4 Page(s): 220-227

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Title

Characterizing and controlling the loading of Vipera albicornuta venom in chitosan nanoparticles as an adjuvant and vaccine delivery system

Author(s)

Keywords

Chitosan

Vipera albicornuta venom

Nanoparticles

Antigen delivery

Abstract

The purpose of this research is designing and preparing the Chitosan Nanoparticles (CS-NPs) loading with snake (Vipera albicornuta) venom as well as evaluating the influence of different factors on the encapsulation efficiency (EE) and loading capacity (LC) of venom. The morphologies and characteristics of CS-NPs were specified respectively through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). CS-NPs were fabricated based on the ionic gelation method of tripolyphosphate (TPP) and Chitosan (CS). The results showed a smooth surface, spherical shape by SEM. The particle size of loaded nanoparticle was 187 nm and zeta potential was found to be 46. 7 mV. Optimum concentration obtained 400 μ g/ml for loading of venom, which leads to LC 86% and EE 91%. Based on the results of the study, it can be concluded that the Vipera albicornuta venom loaded CS-NPs may be used as a new Antigen delivery system.

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APA: Copy

Shambayati, Mohammad Hassan, MEHRABI, MOHSEN, Mohammadpour Dounighi, Nasser, RAMAZANI, ALI, ZARE MIRAKABADI, ABBAS, & AHMADI, EBRAHIM. (2019). Characterizing and controlling the loading of Vipera albicornuta venom in chitosan nanoparticles as an adjuvant and vaccine delivery system. NANOMEDICINE RESEARCH JOURNAL, 4(4), 220-227. SID. https://sid.ir/paper/754571/en

Vancouver: Copy

Shambayati Mohammad Hassan, MEHRABI MOHSEN, Mohammadpour Dounighi Nasser, RAMAZANI ALI, ZARE MIRAKABADI ABBAS, AHMADI EBRAHIM. Characterizing and controlling the loading of Vipera albicornuta venom in chitosan nanoparticles as an adjuvant and vaccine delivery system. NANOMEDICINE RESEARCH JOURNAL[Internet]. 2019;4(4):220-227. Available from: https://sid.ir/paper/754571/en

IEEE: Copy

Mohammad Hassan Shambayati, MOHSEN MEHRABI, Nasser Mohammadpour Dounighi, ALI RAMAZANI, ABBAS ZARE MIRAKABADI, and EBRAHIM AHMADI, “Characterizing and controlling the loading of Vipera albicornuta venom in chitosan nanoparticles as an adjuvant and vaccine delivery system,” NANOMEDICINE RESEARCH JOURNAL, vol. 4, no. 4, pp. 220–227, 2019, [Online]. Available: https://sid.ir/paper/754571/en

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