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Information Journal Paper

Title

Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study

Pages

  53-58

Abstract

 Introduction: Cutaneous leishmaniasis (CL) is one of the infectious diseases and health problems in tropical regions. Glucantime is commonly used to treat CL and it, not only has some side effects but also observation shows the drug resistance of some of the various Leishmania species. Therefore, the aim of this study was to investigate the effect of silver nanoparticles (AgNPs) and bioresonance waves on Leishmania, in vitro. Materials and Methods: In the present experimental study, Leishmania major promastigotes were cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin and streptomycin at 23° C. After 6 days, the parasites achieved stationary phases of promastigotes, Then the effects of different concentrations of AgNPs (1, 3, 5, 10 and 25 μ g/mL) and different radiation times of bioresonance wave (5 and 20 minutes) were investigated. Herein, the effects of various treatment on parasites proliferation were evaluated with live promastigotes counting after 24, 48 and 72 hours treatment. Results: The parasite count showed that the various concentrations of AgNPs, radiation of bioresonance wave and combination significantly decreased the numbers of live promastigotes over time compared with the control group after 72 hours. The highest antileishmanial activity was seen for AgNPs at concentration of 1 μ g/mL when combined with 20 minutes radiation of bioresonance wave (proliferation inhibition: 79. 92%) Conclusions: Based on our result, AgNPs and bioresonance waves are potent antileishmanial agents. The authors declare that the more studies should be done.

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    APA: Copy

    Azimijou, Nayereh, KESHVARI, HAMID, SEYYED TABAEI, SEYYED JAVAD, RAHIMI, MOHSEN, & IMANZADEH, MEHRDAD. (2020). Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, 7(1), 53-58. SID. https://sid.ir/paper/759740/en

    Vancouver: Copy

    Azimijou Nayereh, KESHVARI HAMID, SEYYED TABAEI SEYYED JAVAD, RAHIMI MOHSEN, IMANZADEH MEHRDAD. Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS[Internet]. 2020;7(1):53-58. Available from: https://sid.ir/paper/759740/en

    IEEE: Copy

    Nayereh Azimijou, HAMID KESHVARI, SEYYED JAVAD SEYYED TABAEI, MOHSEN RAHIMI, and MEHRDAD IMANZADEH, “Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study,” JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, vol. 7, no. 1, pp. 53–58, 2020, [Online]. Available: https://sid.ir/paper/759740/en

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