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Information Journal Paper

Title

Assay of Bacteriorhodopsin Activity and Structure on Polycarbonate Surface by Spin Coating Method and Photochemical Activity Analysis

Pages

  6-12

Abstract

 Introduction: Protein-based memory is a novel technology that employs proteins ability to participate in electronic processes. Bacteriorhodopsin (BR) is a membranous proton pump that it’ s applications in bio-molecular electronic devices has been widely studied. The results of this research show that BR bounded to modified Polycarbonate Surface has higher activity for Spin Coating method. Materials and Methods: In an in-vitro study, BR-containing polymer matrix of polyvinyl alcohol and gelatin with different w/v ratios was prepared. Spectroscopic and enzyme activity analysis was performed and the optimized concentration for BR-containing films was determined to be 3. 2 mg/ml. BRpolymer was then immobilized on the Polycarbonate Surface with Spin Coating method and AFM microscopy was used to characterize BR-coated polycarbonate. Results: Based on the obtained results we conclude that polymer concentrations below 1% significantly reduced BR activity levels. A280/A570 of 3. 64 for 3. 2 mg/ml BR solution and 4. 97 for BR in 1% polymer confirmed the quality prepared film. AFM study of BR-coated Polycarbonate Surface revealed the overall thickness of 25nm, indicating that we were able to prepare a surface with suitable thickness for nano electronic devices Conclusion: The results of this research show that BR bounded to modified Polycarbonate Surface has higher activity for Spin Coating method.

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    Cite

    APA: Copy

    Nazarian, Niloofar, Molai Rad, Ahmad, POOLADI, MEHDI, & Taghizadeh, Bita. (2019). Assay of Bacteriorhodopsin Activity and Structure on Polycarbonate Surface by Spin Coating Method and Photochemical Activity Analysis. ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES), 10(3), 6-12. SID. https://sid.ir/paper/761732/en

    Vancouver: Copy

    Nazarian Niloofar, Molai Rad Ahmad, POOLADI MEHDI, Taghizadeh Bita. Assay of Bacteriorhodopsin Activity and Structure on Polycarbonate Surface by Spin Coating Method and Photochemical Activity Analysis. ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES)[Internet]. 2019;10(3):6-12. Available from: https://sid.ir/paper/761732/en

    IEEE: Copy

    Niloofar Nazarian, Ahmad Molai Rad, MEHDI POOLADI, and Bita Taghizadeh, “Assay of Bacteriorhodopsin Activity and Structure on Polycarbonate Surface by Spin Coating Method and Photochemical Activity Analysis,” ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES), vol. 10, no. 3, pp. 6–12, 2019, [Online]. Available: https://sid.ir/paper/761732/en

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