PURIFICATION OF KUNITZ SOYBEAN TRYPSIN INHIBITOR USING AFFINITY CHROMATHOGRAPHY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAKIBA Y.A.; MOSTAFAEI ALI; PARVANEH SH.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Scientific Journal of Kurdistan University of Medical Sciences Year:2007 | Volume:12 | Issue:2 (SERIAL NUMBER 44) Page(s): 77-83

Download Full-Text

Persian Verion

View:

2,515

Download:

Cites:

Information Journal Paper

Title

PURIFICATION OF KUNITZ SOYBEAN TRYPSIN INHIBITOR USING AFFINITY CHROMATHOGRAPHY

Author(s)

SHAKIBA Y.A. | MOSTAFAEI ALI | PARVANEH SH. | Issue Writer Certificate

Keywords

SOYBEANQ3

TRYPSIN INHIBITORQ3

TRYPSINQ3

AFFINITY CHROMATOGRAPHYQ3

Abstract

Background and aim: SOYBEAN TRYPSIN INHIBITOR/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> TRYPSIN INHIBITOR, a single chain protein with 181 amino acids and two disulfide bonds, has special tendency for pancreatic TRYPSIN enzyme. This protein exists in SOYBEAN abundantly and shows high resistance to heating and chemicals. Various procedures such as combination of different chromatographic methods, immobilized metal AFFINITY CHROMATOGRAPHY and preparative electrophoresis have been used to purify this protein.Materials and Methods: Pulverized SOYBEAN was defatted with methanol and the reminder was extracted after being solved in dionized water. Storage proteins in solution were eliminated by use of isoelelctric precipitation method. TRYPSIN INHIBITOR/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> TRYPSIN INHIBITOR riched fraction was adsorbed on an affinity column with TRYPSIN as the ligand. After washing unbonded proteins, TRYPSIN INHIBITOR/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> TRYPSIN INHIBITOR was eluted from the collumn by decreasing pH. Purity and activity of the inhibitor was assessed by SDS-PAGE and a spectrophotometric method, respectively.Results: SDS-PAGE demonstrated a protein with a single band and molecular weight of 20 KDa with purity of more than 99 percent under reducing and non-reducing conditions. Activity of the purified inhibitor protein was 3600 TIU/mg. The results showed that overloading of the affinity collumn to gether with chromatography at pH of 6 to 8 increased the purity.Conclusion: The proposed method is simple and efficient for preparation of large amounts of pure TRYPSIN INHIBITOR/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> TRYPSIN INHIBITOR. In addition, this method does not need any further steps such as dialysis.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAKIBA, Y.A., MOSTAFAEI, ALI, & PARVANEH, SH.. (2007). PURIFICATION OF KUNITZ SOYBEAN TRYPSIN INHIBITOR USING AFFINITY CHROMATHOGRAPHY. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 12(2 (SERIAL NUMBER 44)), 77-83. SID. https://sid.ir/paper/76367/en

Vancouver: Copy

SHAKIBA Y.A., MOSTAFAEI ALI, PARVANEH SH.. PURIFICATION OF KUNITZ SOYBEAN TRYPSIN INHIBITOR USING AFFINITY CHROMATHOGRAPHY. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2007;12(2 (SERIAL NUMBER 44)):77-83. Available from: https://sid.ir/paper/76367/en

IEEE: Copy

Y.A. SHAKIBA, ALI MOSTAFAEI, and SH. PARVANEH, “PURIFICATION OF KUNITZ SOYBEAN TRYPSIN INHIBITOR USING AFFINITY CHROMATHOGRAPHY,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 12, no. 2 (SERIAL NUMBER 44), pp. 77–83, 2007, [Online]. Available: https://sid.ir/paper/76367/en

Related Journal Papers