Cytotoxic and apoptotic effects of neurokinin-1 receptor (NK1R) antagonist on multiple myeloma cells

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Razani Elham; BASHASH DAVOOD

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Journal: SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES Year:2020 | Volume:24 | Issue:5 (103) Page(s): 1-11

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Title

Cytotoxic and apoptotic effects of neurokinin-1 receptor (NK1R) antagonist on multiple myeloma cells

Author(s)

Razani Elham | BASHASH DAVOOD | Issue Writer Certificate

Keywords

Multiple myelomaQ1

NK1RQ1

AprepitantQ1

KMM-1 cell lineQ1

Abstract

Background and Aim: Despite therapeutic improvements in recent decades, Multiple myeloma (MM) still remains as one of the leading causes of death all over the world. Previous studies have indicated role of neurokinin-1 receptor (NK1R) in the pathogenesis of cancer. Therefore, we decided to evaluate cytotoxic and apoptotic effects of Aprepitant (NK1R antagonist) on MM-derived KMM-1 cell line. Material and Method: In this experimental study, for assessment of cytotoxic effect of Aprepitant on MM cells, KMM-1 (prepared in Tarbiat Modarres University) were exposed to different concentrations of the inhibitor (5, 15, 30 and 45 μ M) and subsequently viability, cell count, metabolic activity, and induction of apoptosis were investigated using trypan blue, MTT, and annexin/PI staining assays respectively. Moreover, in order to examine the molecular mechanism of action of Aprepitant in KMM-1 cells, gene expression analysis was performed by real-time PCR. Results: The results indicated that NK1R inhibition using Aprepitant resulted in considerable growth suppression of KMM-1 cells. Moreover, we found that the cytotoxic effects were likely due to cell arrest in G1 phase and induction of apoptosis, as revealed by the increased percentage of annexin-V/PI double positive cells among the inhibitor-treated cells (P<0. 05). Also, RQ-PCR analysis revealed Aprepitant changed the ratio of expression of pro apoptotic to anti apoptotic genes by way of alteration in the expression levels of Bax and Bcl-2 (P<0. 05). Conclusion: The present study highlighted the efficiency of Aprepitant in suppression of the growth of KMM-1 cells; however, further studies are needed to determine the safety and efficacy of this agent for the treatment of MM patients.

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APA: Copy

Razani, Elham, & BASHASH, DAVOOD. (2020). Cytotoxic and apoptotic effects of neurokinin-1 receptor (NK1R) antagonist on multiple myeloma cells. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 24(5 (103) ), 1-11. SID. https://sid.ir/paper/76427/en

Vancouver: Copy

Razani Elham, BASHASH DAVOOD. Cytotoxic and apoptotic effects of neurokinin-1 receptor (NK1R) antagonist on multiple myeloma cells. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;24(5 (103) ):1-11. Available from: https://sid.ir/paper/76427/en

IEEE: Copy

Elham Razani, and DAVOOD BASHASH, “Cytotoxic and apoptotic effects of neurokinin-1 receptor (NK1R) antagonist on multiple myeloma cells,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 5 (103) , pp. 1–11, 2020, [Online]. Available: https://sid.ir/paper/76427/en

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