ISOLATION AND PRIMARY CULTURE OF RAT THYMIC EPITHELIAL CELLS USING ACTINIDIN ENZYME OF KIWI FRUIT

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MANSOURI K.; MOSTAFAEI ALI; SHIRVANI Z.; BIDMESHKIPOUR A.

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Journal: SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES Year:2008 | Volume:13 | Issue:1 (SERIAL NUMBER 47) Page(s): 36-43

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Title

ISOLATION AND PRIMARY CULTURE OF RAT THYMIC EPITHELIAL CELLS USING ACTINIDIN ENZYME OF KIWI FRUIT

Author(s)

MANSOURI K. | MOSTAFAEI ALI | SHIRVANI Z. | BIDMESHKIPOUR A. | Issue Writer Certificate

Keywords

ACTINIDINQ3

THYMIC EPITHELIAL CELLSQ3

PRIMARY CULTUREQ3

COLLAGENASEQ3

Abstract

Background and Aim: Proteolytic enzymes specially COLLAGENASE are used for degredation of extracellular matrix, cell isolation and primary cell culture. It is important to substitute a low cost and easily obtained plant or animal protease for COLLAGENASE. In the present study the enzyme ACTINIDIN which is found abundantly in kiwi fruit, was used to isolate THYMIC EPITHELIAL CELLS from rat thymus. Material and Methods: ACTINIDIN with different concentrations (from 1 to 10 mg/ml) and at different times (3, 4 or 5h) was used to isolat rat THYMIC EPITHELIAL CELLS. The isolated epithelial cells were cultured on collagen coated dishes in willam's E medium. The percentage of viable isolated cells was estimated by the trypan blue test and morphology of the cells examined microscopically after staining with Papanicoloau.Results: ACTINIDIN with a concentration of 4 mg/ml for 3.5-4 h digested extra-cellular matrix of rat thymus and isolated thymic epithelial cell appropriately. The rate of viability of the separated cells was estimated 90-95% in all isolates.Conclusion: The results of this study indicated ACTINIDIN is comparable to COLLAGENASE in isolation of epithelial cells from the thymus of rat and probably other animals.Considering its simpler purification and its low cost, this enzyme is a suitable and safe substitute for COLLAGENASE which can be used for isolation of THYMIC EPITHELIAL CELLS from rat and probably other animals.

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References

KIWIFRUIT ACTINIDIN: ITS PURIFICATION AND EXAMINATION OF AMOUNT IN DIFFERENT VARIETIES

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APA: Copy

MANSOURI, K., MOSTAFAEI, ALI, SHIRVANI, Z., & BIDMESHKIPOUR, A.. (2008). ISOLATION AND PRIMARY CULTURE OF RAT THYMIC EPITHELIAL CELLS USING ACTINIDIN ENZYME OF KIWI FRUIT. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 13(1 (SERIAL NUMBER 47)), 36-43. SID. https://sid.ir/paper/76549/en

Vancouver: Copy

MANSOURI K., MOSTAFAEI ALI, SHIRVANI Z., BIDMESHKIPOUR A.. ISOLATION AND PRIMARY CULTURE OF RAT THYMIC EPITHELIAL CELLS USING ACTINIDIN ENZYME OF KIWI FRUIT. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2008;13(1 (SERIAL NUMBER 47)):36-43. Available from: https://sid.ir/paper/76549/en

IEEE: Copy

K. MANSOURI, ALI MOSTAFAEI, Z. SHIRVANI, and A. BIDMESHKIPOUR, “ISOLATION AND PRIMARY CULTURE OF RAT THYMIC EPITHELIAL CELLS USING ACTINIDIN ENZYME OF KIWI FRUIT,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 13, no. 1 (SERIAL NUMBER 47), pp. 36–43, 2008, [Online]. Available: https://sid.ir/paper/76549/en

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