EVALUATTION OF GIARDIA LAMBLIA GENETIC DIFFERENCES IN KHORRAMABAD CITY AND SURROUNDING VILLAGES BY USE OF PCR AND SEQUENCING

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AKBARIAN AMIN; SADRAEI JAVID; FOROUZANDEH MEHDI

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Paper Information

Journal: Scientific Journal of Kurdistan University of Medical Sciences Year:2012 | Volume:17 | Issue:2 (SERIAL NUMBER 64) Page(s): 61-71

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Information Journal Paper

Title

EVALUATTION OF GIARDIA LAMBLIA GENETIC DIFFERENCES IN KHORRAMABAD CITY AND SURROUNDING VILLAGES BY USE OF PCR AND SEQUENCING

Author(s)

AKBARIAN AMIN | SADRAEI JAVID | FOROUZANDEH MEHDI | Issue Writer Certificate

Keywords

GENOTYPEQ2

GIARDIA LAMBLIAQ3

KHORRAMABAD CITYQ2

PCR AND SEQUENCINGQ2

Abstract

Background and Aim: The intestinal protozoa GIARDIA LAMBLIA is a parasite frequently involved in human parasitic gastroenteritis throughout the world. Transmission of G. lamblia cysts to human occurs mainly via ingestion of contaminated food and water. The aim of this study was to evaluate GIARDIA LAMBLIA genetic differences in the KHORRAMABAD CITY and its surrounding villages by means of PCR AND SEQUENCING.Material and Methods: In these study 30 fecal samples positive for GIARDIA LAMBLIA were collected from the patients in KHORRAMABAD CITY and its surrounding villages. The samples were fixed in dichromate 5% after filtration. Before DNA extraction, all samples were washed with PBS solution in order to remove dichromate. For determination of genetic differences sequencing on 5 samples was performed.Results: After DNA extraction, amplification of GDH gene from 24 of 30 samples was performed by PCR, successfully. Alignment of the obtained GDH sequences with reference sequences (gene bank) indicated the presence of only one GENOTYPE of G. lamblia; 5 specimens were identified as G. lamblia assemblage A.Conclusion: Assemblage A was the dominant GENOTYPE in KHORRAMABAD CITY and its surrounding villages. Because of limited number of samples in this study, further studies with higher number of samples are recommended.

Cites

GENOTYPING OF GIARDIA DUODENALIS BY β-GIARDIN GENE IN ASYMPTOMATIC PATIENTS

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APA: Copy

AKBARIAN, AMIN, SADRAEI, JAVID, & FOROUZANDEH, MEHDI. (2012). EVALUATTION OF GIARDIA LAMBLIA GENETIC DIFFERENCES IN KHORRAMABAD CITY AND SURROUNDING VILLAGES BY USE OF PCR AND SEQUENCING. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 17(2 (SERIAL NUMBER 64)), 61-71. SID. https://sid.ir/paper/76789/en

Vancouver: Copy

AKBARIAN AMIN, SADRAEI JAVID, FOROUZANDEH MEHDI. EVALUATTION OF GIARDIA LAMBLIA GENETIC DIFFERENCES IN KHORRAMABAD CITY AND SURROUNDING VILLAGES BY USE OF PCR AND SEQUENCING. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;17(2 (SERIAL NUMBER 64)):61-71. Available from: https://sid.ir/paper/76789/en

IEEE: Copy

AMIN AKBARIAN, JAVID SADRAEI, and MEHDI FOROUZANDEH, “EVALUATTION OF GIARDIA LAMBLIA GENETIC DIFFERENCES IN KHORRAMABAD CITY AND SURROUNDING VILLAGES BY USE OF PCR AND SEQUENCING,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 17, no. 2 (SERIAL NUMBER 64), pp. 61–71, 2012, [Online]. Available: https://sid.ir/paper/76789/en

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