DETERMINATION OF PHYLOGENETIC RELATIONSHIP AMONG VIBRIO PARAHAEMOLYTICUS ISOLATES FROM PERSIAN GULF AND THE EVALUATION OF THEIR SUSCEPTIBILITY TO ANTIBIOTICS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAGH NEGAHDAR S.; BASERI SALEHI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Armaghane Danesh Year:2016 | Volume:21 | Issue:6 (113) Page(s): 605-616

Download Full-Text

Persian Verion

View:

763

Download:

Cites:

Information Journal Paper

Title

DETERMINATION OF PHYLOGENETIC RELATIONSHIP AMONG VIBRIO PARAHAEMOLYTICUS ISOLATES FROM PERSIAN GULF AND THE EVALUATION OF THEIR SUSCEPTIBILITY TO ANTIBIOTICS

Author(s)

HAGH NEGAHDAR S. | BASERI SALEHI M. | Issue Writer Certificate

Keywords

VIBRIO PARAHAEMOLYTICUSQ1

PHYLOGENETIC RELATIONSHIPSQ1

ANTIBIOTIC SUSCEPTIBILITYQ1

16SRRNAQ2

PERSIAN GOLFQ2

Abstract

Background & aim: VIBRIO PARAHAEMOLYTICUS is a Gram negative halophilic bacterium found in aquatic environments. This bacterium has been introduced as a cause of acute gastroenteritis following the consumption of raw and uncooked seafood. Major symptoms of VIBRIO PARAHAEMOLYTICUS illness is watery diarrhea, abdominal cramps, Nausea, Vomiting, Fever, Headache and Bloody diarrhea. The purpose of this study was isolation and identification of VIBRIO PARAHAEMOLYTICUS from Persian Gulf and the determination of their phylogenetic relationship. In addition, ANTIBIOTIC SUSCEPTIBILITY of the isolates was evaluated in order to achieve maximum information concerning a drug of choice.Methods: In this study, 89 samples of water, sediments, fish and shrimp were collected from different regions of the Persian Gulf. All samples were asssessed for phenotypic and molecular isolation and identification of VIBRIO PARAHAEMOLYTICUS. Then the positive and negative strains of conagua VIBRIO PARAHAEMOLYTICUS were determined based on hemolysin production. the identification and phylogenetic relationship were analyzed using the mega-6 software. Finally susceptibility of Kanagawa positive VIBRIO PARAHAEMOLYTICUS to antibiotics was evaluated by disk diffusion method and minimal inhibitory concentrations of effective antibiotics were assessed using double serial dilution method.Results: A total of nine VIBRIO PARAHAEMOLYTICUS were isolated and identified. Of all isolates five strains were Kanagawa positive and four were Kanagawa negative. All isolates exhibited phylogenetic relationship to each other except one strain (NSP1). The results obtained from ANTIBIOTIC SUSCEPTIBILITY of Kanagawa positive VIBRIO PARAHAEMOLYTICUS isolates illustrated that most of the isolates were resistance to Vancomycin, Oxacillin, and Amikacin and susceptible to Trimetoprim Sulfometaxazole respectively. In addition, the lowest Minimal inhibitory Concentration (MIC) value was found for Sulfometaxazole and highest MIC value was found for Nitofuran.Conclusion: The present study illustrated that both pathogenic and nonpathogenic VIBRIO PARAHAEMOLYTICUS existed in the persian Gulf. On the other hand these bacteria that could facilitate phylogenetic relationship with each other because of horizontal gene transfer among them.Trimetoprim Sulfometaxazole could be considered a durg of choice for treatment of the patient suffering from VIBRIO PARAHAEMOLYTICUS infections.

Cites

No record.

References

No record.

Cite

APA: Copy

HAGH NEGAHDAR, S., & BASERI SALEHI, M.. (2016). DETERMINATION OF PHYLOGENETIC RELATIONSHIP AMONG VIBRIO PARAHAEMOLYTICUS ISOLATES FROM PERSIAN GULF AND THE EVALUATION OF THEIR SUSCEPTIBILITY TO ANTIBIOTICS. ARMAGHAN DANESH, 21(6 (113)), 605-616. SID. https://sid.ir/paper/77615/en

Vancouver: Copy

HAGH NEGAHDAR S., BASERI SALEHI M.. DETERMINATION OF PHYLOGENETIC RELATIONSHIP AMONG VIBRIO PARAHAEMOLYTICUS ISOLATES FROM PERSIAN GULF AND THE EVALUATION OF THEIR SUSCEPTIBILITY TO ANTIBIOTICS. ARMAGHAN DANESH[Internet]. 2016;21(6 (113)):605-616. Available from: https://sid.ir/paper/77615/en

IEEE: Copy

S. HAGH NEGAHDAR, and M. BASERI SALEHI, “DETERMINATION OF PHYLOGENETIC RELATIONSHIP AMONG VIBRIO PARAHAEMOLYTICUS ISOLATES FROM PERSIAN GULF AND THE EVALUATION OF THEIR SUSCEPTIBILITY TO ANTIBIOTICS,” ARMAGHAN DANESH, vol. 21, no. 6 (113), pp. 605–616, 2016, [Online]. Available: https://sid.ir/paper/77615/en

Related Journal Papers