EVALUATION OF SUSCEPTIBILITY OF STRAINS OF CANDIDA ALBICANS ISOLATED FROM AIDS PATIENTS TO FLUCONAZOLE AND DETERMINATION OF CDR2 RESISTANCE GENE IN RESISTANT STRAINS BY RT-PCR METHOD

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FARAHBAKHSH E.; YADEGARI M.H.; RAJABI BAZL M.; TAGHIZADEH ARMAKI M.

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Journal: Armaghane Danesh Year:2011 | Volume:16 | Issue:3 (63) Page(s): 201-210

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Title

EVALUATION OF SUSCEPTIBILITY OF STRAINS OF CANDIDA ALBICANS ISOLATED FROM AIDS PATIENTS TO FLUCONAZOLE AND DETERMINATION OF CDR2 RESISTANCE GENE IN RESISTANT STRAINS BY RT-PCR METHOD

Author(s)

FARAHBAKHSH E. | YADEGARI M.H. | RAJABI BAZL M. | TAGHIZADEH ARMAKI M. | Issue Writer Certificate

Keywords

CANDIDA ALBICANSQ2

CDR2 GENEQ2

RT-PCRQ2

Abstract

Introduction & Objective: Nowadays, opportunistic fungi especially CANDIDA ALBICANS are the most common cause of life threatening infections in immunodeficiency patients. Increasing Azole-resistant strains of C. albicans are a main problem in human immunodeficiency virus-infected patients. The aim of this study was to evaluate the CDR2 GENE in C. albicans azole resistant strains, isolated from AIDS patients with oropharyngeal candidiasis by RT-PCR method.Materials & Methods: The present experimental study was conducted at Tarbiat Modares University of Medical Sciences in 2009. C. albicans isolates from HIV infected patients were identified by standard procedures, including germ tube formation, clamidospor and color of colonies on CHROM agar. At first, susceptibility of C. albicans isolates was assessed by disk diffusion agar technique. Then, CDR2 resistance gene was analyzed by RTPCR and electrophoresis of the PCR products. Finally, patterns of the resulted bands were compared with standard fluconazole resistant strains. The collected data was analyzed using the SPSS software.Results: The results of drug sensitivity of 66 C. albicans isolates from AIDS patients revealed that 62.6% were susceptible, 8.6% were susceptible-dose dependent (SDD) and 28.7% were resistant. In RT-PCR analysis, 6% of patients had the CDR2 GENE.Conclusion: The use of phenotypic methods like disk diffusion agar, which is cheaper, along with genotypic methods, like RTPCR, which provide the possibility of studying the mechanism of drug resistance, is recommended.

Cites

A SURVEY OF THE EFFECT OF CAMPHOR ON INT1 AND EFG1 GENE EXPRESSIONS OF CANDIDA ALBICANS AT THREE TREATMENT TIMES (24, 48, AND 72 HOURS) VIA REAL-TIME PCR

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APA: Copy

FARAHBAKHSH, E., YADEGARI, M.H., RAJABI BAZL, M., & TAGHIZADEH ARMAKI, M.. (2011). EVALUATION OF SUSCEPTIBILITY OF STRAINS OF CANDIDA ALBICANS ISOLATED FROM AIDS PATIENTS TO FLUCONAZOLE AND DETERMINATION OF CDR2 RESISTANCE GENE IN RESISTANT STRAINS BY RT-PCR METHOD. ARMAGHAN DANESH, 16(3 (63)), 201-210. SID. https://sid.ir/paper/77963/en

Vancouver: Copy

FARAHBAKHSH E., YADEGARI M.H., RAJABI BAZL M., TAGHIZADEH ARMAKI M.. EVALUATION OF SUSCEPTIBILITY OF STRAINS OF CANDIDA ALBICANS ISOLATED FROM AIDS PATIENTS TO FLUCONAZOLE AND DETERMINATION OF CDR2 RESISTANCE GENE IN RESISTANT STRAINS BY RT-PCR METHOD. ARMAGHAN DANESH[Internet]. 2011;16(3 (63)):201-210. Available from: https://sid.ir/paper/77963/en

IEEE: Copy

E. FARAHBAKHSH, M.H. YADEGARI, M. RAJABI BAZL, and M. TAGHIZADEH ARMAKI, “EVALUATION OF SUSCEPTIBILITY OF STRAINS OF CANDIDA ALBICANS ISOLATED FROM AIDS PATIENTS TO FLUCONAZOLE AND DETERMINATION OF CDR2 RESISTANCE GENE IN RESISTANT STRAINS BY RT-PCR METHOD,” ARMAGHAN DANESH, vol. 16, no. 3 (63), pp. 201–210, 2011, [Online]. Available: https://sid.ir/paper/77963/en

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