ANTIVIRAL EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF ROSMARINUS OFFICINALIS AGAINST HERPES SIMPLEX VIRUS TYPE 1 IN HELA CELL CULTURE

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YOUSEFI KORDESTANI G.; PARSANIA M.

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Journal: Armaghane Danesh Year:2015 | Volume:20 | Issue:8 (103) Page(s): 706-719

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Title

ANTIVIRAL EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF ROSMARINUS OFFICINALIS AGAINST HERPES SIMPLEX VIRUS TYPE 1 IN HELA CELL CULTURE

Author(s)

YOUSEFI KORDESTANI G. | PARSANIA M. | Issue Writer Certificate

Keywords

ROSMARINUS OFFICINALISQ2

HERPES SIMPLEX VIRUS TYPE 1Q3

HELA CELL CULTUREQ3

Abstract

Background & aim: HERPES SIMPLEX VIRUS TYPE 1 (HSV-1) is responsible for many diseases such as herpes labialis, conjunctivitis, encephalitis, and infections of newborns. Several antiviral drugs including acyclovir, penciclovir and valacyclovir which inhibit the virus-specific enzymes such as DNA polymerase have been used against HSV-1 infections. Occurrence of infections caused by Herpes simplex virus and also the growing rate of virus resistance to common drug has increased. ROSMARINUS OFFICINALIS with the scientific name (ROSMARINUS OFFICINALIS) has several compounds with antibacterial properties, antioxidant and anti-fungal properties. The aim of this study was to investigate the antiviral effect of rosemary extract on herpes simplex virus type one in HeLa cell.Methods: In the present experimental study, the cytotoxic effect of hydro-alcoholic extract of R. officinalis in different concentrations at 2-0.1 mg/ml was determined on Hela cell line monolayer by trypan blue and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) methods. The maximum range of non-toxic concentration of R. officinalis extract on Hela cell line was determined. Direct effect of concentration of aforementioned extract was examined in extracellular condition and after that antiviral effect of maximum non-toxic and lower concentrations was evaluated in different periods of virus replication in Hela cell line. Virus titer was measured by tissue culture infectious dose 50 (TCID50) methods.Results: The results showed that the hydro-alcoholic extract of R.officinalis at the concentration of 0.6 mg/ml was determined as non-toxic on Hela cell line .This concentration did not have significant virucidal effects on Herpes simplex virus. This concentration had maximum antiviral effects on virus replication. The maximum antiviral effects of extract were exhibited immediately after virus adsorption and 1 hour after cells infection. In this condition, the virus titer decreased significantly compared to control and other times of virus replication.Conclusion: In accordance with the results of the present study, photochemical constituents in hydro-alcoholic extract of R. officinalis had predominant effects on early stage of viral replication and it seems that R. officinalis had therapeutic effects on herpes simplex virus infections.

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APA: Copy

YOUSEFI KORDESTANI, G., & PARSANIA, M.. (2015). ANTIVIRAL EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF ROSMARINUS OFFICINALIS AGAINST HERPES SIMPLEX VIRUS TYPE 1 IN HELA CELL CULTURE. ARMAGHAN DANESH, 20(8 (103)), 706-719. SID. https://sid.ir/paper/78176/en

Vancouver: Copy

YOUSEFI KORDESTANI G., PARSANIA M.. ANTIVIRAL EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF ROSMARINUS OFFICINALIS AGAINST HERPES SIMPLEX VIRUS TYPE 1 IN HELA CELL CULTURE. ARMAGHAN DANESH[Internet]. 2015;20(8 (103)):706-719. Available from: https://sid.ir/paper/78176/en

IEEE: Copy

G. YOUSEFI KORDESTANI, and M. PARSANIA, “ANTIVIRAL EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF ROSMARINUS OFFICINALIS AGAINST HERPES SIMPLEX VIRUS TYPE 1 IN HELA CELL CULTURE,” ARMAGHAN DANESH, vol. 20, no. 8 (103), pp. 706–719, 2015, [Online]. Available: https://sid.ir/paper/78176/en

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