CYTOTOXIC EVALUATION OF STXA1-GM-CSF RECOMBINANT HYBRID ON DIFFERENT CANCER CELL LINES EXPRESSING GM-CSF RECEPTOR

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HABIBI ROUDKENAR M.; OLOUMI MANA; SHOKRGOZAR M.A.; SHAHROKHI N.; MOHAMMADI ROUSHANDEH A.; KUWAHARA Y.; FUKUMOTO M.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2006 | Volume:3 | Issue:1 Page(s): 1-8

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Title

CYTOTOXIC EVALUATION OF STXA1-GM-CSF RECOMBINANT HYBRID ON DIFFERENT CANCER CELL LINES EXPRESSING GM-CSF RECEPTOR

Author(s)

Keywords

SHIGA-TOXINQ4

GRANULOCYTE MACROPHAGE - COLONY STIMULATING FACTOR (GM–CSF)Q4

CYTOTOXICITYQ4

CANCER CELLSQ4

Abstract

Background and ObjectivesImmunotoxins are comprised of cell targeting and cell killing moieties. Immunotoxins have been proposed as new strategy for cancer treatment. In this study, catalytic domain of Shigatoxin (A1) was fused to human Granulocyte Macrophage-Colony Stimulating Factor (h GM-CSF). The fused gene was already expressed in E.coli and the expressed protein was analysed for its cytotoxic activity on human cancer cell lines expressing GM-CSF receptor (GM-CSFR). Moreover, the impact of GM-CSF receptor on inhibition of cytotoxic effect of SHIGA-TOXIN-GM-CSF recombinant hybrid in cell lines expressing GM-CSF receptor (GMCSFR) was evaluated.Materials and MethodsCell lines were grown in RPMI-1640 medium supplemented with 10-20% heat inactivated fetal bovine serum (Gibco-BRL). Cytotoxic activity was checked on the cell lines and was measured by trypan blue staininig and MTT assay. GM-CSF receptor was blocked by anti-GM-CSF antibody.ResultsCytotoxicity studies revealed that the chimeric protein induced cytotoxic effect on different cell lines. This effect was found to be specific due to the presence of killing moiety (A1) that exerts its effect through specific cell targeting domain i.e. GM-CSF, by binding to its receptor present on those cell lines. K562 and LS174T were the most sensitive.Conclusions Our results revealed that the hybrid protein is toxic to the cancer cell lines bearing GM-CSF receptor; this effect could be a potential factor for further consideration of hybrid protein as a therapeutic agent.

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APA: Copy

HABIBI ROUDKENAR, M., OLOUMI, MANA, SHOKRGOZAR, M.A., SHAHROKHI, N., MOHAMMADI ROUSHANDEH, A., KUWAHARA, Y., & FUKUMOTO, M.. (2006). CYTOTOXIC EVALUATION OF STXA1-GM-CSF RECOMBINANT HYBRID ON DIFFERENT CANCER CELL LINES EXPRESSING GM-CSF RECEPTOR. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 3(1), 1-8. SID. https://sid.ir/paper/78450/en

Vancouver: Copy

HABIBI ROUDKENAR M., OLOUMI MANA, SHOKRGOZAR M.A., SHAHROKHI N., MOHAMMADI ROUSHANDEH A., KUWAHARA Y., FUKUMOTO M.. CYTOTOXIC EVALUATION OF STXA1-GM-CSF RECOMBINANT HYBRID ON DIFFERENT CANCER CELL LINES EXPRESSING GM-CSF RECEPTOR. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2006;3(1):1-8. Available from: https://sid.ir/paper/78450/en

IEEE: Copy

M. HABIBI ROUDKENAR, MANA OLOUMI, M.A. SHOKRGOZAR, N. SHAHROKHI, A. MOHAMMADI ROUSHANDEH, Y. KUWAHARA, and M. FUKUMOTO, “CYTOTOXIC EVALUATION OF STXA1-GM-CSF RECOMBINANT HYBRID ON DIFFERENT CANCER CELL LINES EXPRESSING GM-CSF RECEPTOR,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 3, no. 1, pp. 1–8, 2006, [Online]. Available: https://sid.ir/paper/78450/en

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