ANTIBODY SCREENING AND IDENTIFICATION BY GEL METHOD IN THALASSEMIC PATIENTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZAR KEYVAN A.; AHMADI MOHAMMAD HOSSEIN; GHARAH BAGHIAN A.; ZOU ALFAGHARI ANARAKI S.; NASIZADEH S.; MAGHSOUDLOU MAHTAB; TOULABI A.A.M.; LOTFI P.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: The Scientific Journal of Iranian Blood Transfusion Year:2008 | Volume:5 | Issue:2 (19) Page(s): 99-108

Download Full-Text

Persian Verion

View:

3,716

Download:

Cites:

Information Journal Paper

Title

ANTIBODY SCREENING AND IDENTIFICATION BY GEL METHOD IN THALASSEMIC PATIENTS

Author(s)

Keywords

SCREENINGQ2

THALASSEMIAQ3

BLOOD TRANSFUSIONQ2

IMMUNIZATIONQ4

Abstract

Background and Objectives: Thalassemic patients need regular BLOOD TRANSFUSION because of chronic nature of disease. Since just major blood groups are controlled in compatibility tests, formation of antibody against minor blood groups (irregular antibodies) can cause transfusion reactions (delayed hemolytic reaction) and may produce complications for patients. In this study, we decided to perform SCREENING and typing of antibodies with gel method and tried to substitute this modern method for routine tests.Materials and Methods: In a descriptive study 441 THALASSEMIA patients having referred to two thalassemic centers in Tehran and Ghazvin were studied. Data forms about BLOOD TRANSFUSION status were filled out for all patients from whom blood samples were then taken. SCREENING antibody test with tube method was performed for all patients followed by assessment with gel method according to its standard pattern and respective program. Antibody identification test was performed according to the respective program as well. Results of obtained data from forms filled out by patients were assessed with SPSS 11.5 software; chi-square test was done with 95 % confidence interval.Results: 441 patients consisting of 234 males (53.1%) and 207 females (46.9 % ) with the mean age of 22.6 years (SD ± 9.27) participated in the study. Out of the total number of subjects, there are 362 THALASSEMIA major (82.1%) and 79 THALASSEMIA intermedia (17.9%). SCREENING showed 391 patients (88.7%) without versus 50 (11.3) with alloantibodies. From among identified antibodies, anti-Kell and anti-D were present in 28% and 16% of cases respectively; antibodies to other Rh subgroups including c, C, e, E and Cw appeared in 26% of cases. Comparing results of microtube gel method with tube method for SCREENING shows that 26 patients recognized as positive in gel method were negative in tube method.Conclusions: Regarding the alloimmunization frequency in thalassemic patients, it is suggested that RBC phenotype especially subgroups of Rh, Kell, Kidd, and Duffy be checked (in addition to ABO Rh(D) group) for every newly diagnosed THALASSEMIA patient; it is further recommended that in every crossmatch for BLOOD TRANSFUSION. Blood bag be selected based on Rh subgroups and Kell in conformity with patient phenotype; this would decrease alloimmunization by 90%.

Cites

References

No record.

Cite

APA: Copy

AZAR KEYVAN, A., AHMADI, MOHAMMAD HOSSEIN, GHARAH BAGHIAN, A., ZOU ALFAGHARI ANARAKI, S., NASIZADEH, S., MAGHSOUDLOU, MAHTAB, TOULABI, A.A.M., & LOTFI, P.. (2008). ANTIBODY SCREENING AND IDENTIFICATION BY GEL METHOD IN THALASSEMIC PATIENTS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 5(2 (19)), 99-108. SID. https://sid.ir/paper/78545/en

Vancouver: Copy

AZAR KEYVAN A., AHMADI MOHAMMAD HOSSEIN, GHARAH BAGHIAN A., ZOU ALFAGHARI ANARAKI S., NASIZADEH S., MAGHSOUDLOU MAHTAB, TOULABI A.A.M., LOTFI P.. ANTIBODY SCREENING AND IDENTIFICATION BY GEL METHOD IN THALASSEMIC PATIENTS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2008;5(2 (19)):99-108. Available from: https://sid.ir/paper/78545/en

IEEE: Copy

A. AZAR KEYVAN, MOHAMMAD HOSSEIN AHMADI, A. GHARAH BAGHIAN, S. ZOU ALFAGHARI ANARAKI, S. NASIZADEH, MAHTAB MAGHSOUDLOU, A.A.M. TOULABI, and P. LOTFI, “ANTIBODY SCREENING AND IDENTIFICATION BY GEL METHOD IN THALASSEMIC PATIENTS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 5, no. 2 (19), pp. 99–108, 2008, [Online]. Available: https://sid.ir/paper/78545/en

Related Journal Papers