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Information Journal Paper

Title

USE OF A SIMPLE METHOD TO IDENTIFY AFLATOXIN-PRODUCING STRAINS OF ASPERGILLUS FLAVUS AND ASPERGILLUS PARASITICUS BY UV PHOTOGRAPHY

Pages

  103-109

Abstract

 Over a period of one year, 24 soil samples collected from different parts of Abyaneh region in Esphahan were analyzed for the presence of AFLATOXIN producing strains of ASPERGILLUS flavus and A.parasiticus.Twenty-one isolates were identified based on the macroscopic and microscopic criteria. These isolates were present in 50% of the samples and their ability to produce aflaloxin was examined in glucose-yeast extract (GY) agar medium by ultra-violet (UV) photography. In the UV photographs, AFLATOXIN-producing mould colonies (8 isolates) were appeared in gray or black color, whereas AFLATOXIN -nonproducing mould colonies (13 isolates) were appeared in white color. All the UV positive or negative isolates were confirmed by thin-layer chromatographic (TLC) analysis of chloroform extracts obtained from GY agar and yeast extract-sucrose (YES) broth media. Among 8 anatoxin-positive isolates, 3 isolates produced both AFLATOXINs B1 and B2 whereas 5 isolates produced only AFLATOXIN B1.The concentration of AFLATOXIN B1 in the isolates producing AFLATOXIN ranged from 0.45 to 2.31µg/g GY agar medium. The results showed that the UV photography in GY agar medium could be used as a rapid and reliable SCREENING test for identification of the isolates.of A flavus and A parasiticus strains that produce AFLATOXIN.

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    APA: Copy

    SHAMS, M., RAZZAGHI, M., & ALAMEH, A.. (2001). USE OF A SIMPLE METHOD TO IDENTIFY AFLATOXIN-PRODUCING STRAINS OF ASPERGILLUS FLAVUS AND ASPERGILLUS PARASITICUS BY UV PHOTOGRAPHY. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 3(2), 103-109. SID. https://sid.ir/paper/80997/en

    Vancouver: Copy

    SHAMS M., RAZZAGHI M., ALAMEH A.. USE OF A SIMPLE METHOD TO IDENTIFY AFLATOXIN-PRODUCING STRAINS OF ASPERGILLUS FLAVUS AND ASPERGILLUS PARASITICUS BY UV PHOTOGRAPHY. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2001;3(2):103-109. Available from: https://sid.ir/paper/80997/en

    IEEE: Copy

    M. SHAMS, M. RAZZAGHI, and A. ALAMEH, “USE OF A SIMPLE METHOD TO IDENTIFY AFLATOXIN-PRODUCING STRAINS OF ASPERGILLUS FLAVUS AND ASPERGILLUS PARASITICUS BY UV PHOTOGRAPHY,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 3, no. 2, pp. 103–109, 2001, [Online]. Available: https://sid.ir/paper/80997/en

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    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
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