DETECTION AND IDENTIFICATION OF LEISHMANIA PARASITES WITHIN SAND FLIES USING KDNA, RDNA AND CPB LOCI

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OSHAGHI M.A.; MALEKI RAVASAN N.; JAVADIAN EZAT ALDIN; RASI Y.; MOHEBALI M.; SADRAEI J.; ZAREEI Z.A.; MOHTARAMI F.

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Journal: Pathobiology Research Year:2008 | Volume:11 | Issue:1-2 Page(s): 81-89

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Title

DETECTION AND IDENTIFICATION OF LEISHMANIA PARASITES WITHIN SAND FLIES USING KDNA, RDNA AND CPB LOCI

Author(s)

Keywords

KALA-AZAR (YL)Q3

SAND FLYQ3

KINETOPLAST DNAQ3

RDNAQ3

CPBQ3

Abstract

Objective: Molecular epidemiology of Visceral Leishmaniasis (VL) is currently used widely for different objectives such as vector incrimination studies.Materials and Methods: In this study three different loci including KINETOPLAST DNA (kDNA), ribosomal DNA (rDNA), and Cystein protease B (CPB) of Leishmania parasite genome were used for detection and identification of natural infection of sand flies of Germi district of Ardebil province, the most important VL or Kala-azar foci in Iran.Results: The results showed that the three loci of kDNA, RDNA and CPBs are respectively more appropriate for leptomonad infection/initial screening, identification of the L.donovani complex, and discrimination of the species complex. It was also verified that both members of the complex, L. donovani and L. infantum, are present in the study area and are transmitted to the hosts by Phlebotomus perfiliewi trenscaucasicus sand flies.Conclusion: This is the first report on natural infection of sand flies to L. donovani in the country and since the ecology and biology of L. donovani differs extensively from L. infantum, it is necessary to perform further studies to highlight the role of L. donovani in epidemiology of VL in the region and country.

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APA: Copy

OSHAGHI, M.A., MALEKI RAVASAN, N., JAVADIAN, EZAT ALDIN, RASI, Y., MOHEBALI, M., SADRAEI, J., ZAREEI, Z.A., & MOHTARAMI, F.. (2008). DETECTION AND IDENTIFICATION OF LEISHMANIA PARASITES WITHIN SAND FLIES USING KDNA, RDNA AND CPB LOCI. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 11(1-2), 81-89. SID. https://sid.ir/paper/81137/en

Vancouver: Copy

OSHAGHI M.A., MALEKI RAVASAN N., JAVADIAN EZAT ALDIN, RASI Y., MOHEBALI M., SADRAEI J., ZAREEI Z.A., MOHTARAMI F.. DETECTION AND IDENTIFICATION OF LEISHMANIA PARASITES WITHIN SAND FLIES USING KDNA, RDNA AND CPB LOCI. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2008;11(1-2):81-89. Available from: https://sid.ir/paper/81137/en

IEEE: Copy

M.A. OSHAGHI, N. MALEKI RAVASAN, EZAT ALDIN JAVADIAN, Y. RASI, M. MOHEBALI, J. SADRAEI, Z.A. ZAREEI, and F. MOHTARAMI, “DETECTION AND IDENTIFICATION OF LEISHMANIA PARASITES WITHIN SAND FLIES USING KDNA, RDNA AND CPB LOCI,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 11, no. 1-2, pp. 81–89, 2008, [Online]. Available: https://sid.ir/paper/81137/en

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