ANALYSIS OF HLA-G TRANSCRIPTS IN PBMCS OF NORMAL AND LUPUS INDIVIDUALS AND EFFECT OF IFN-γ ON THE EXPRESSION OF HLA-G

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ANSARI MOHAMMAD; ZAVARAN HOSSEINI A.; YARI FATEMEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2010 | Volume:12 | Issue:4 Page(s): 11-18

Download Full-Text

Persian Verion

View:

773

Download:

Cites:

Information Journal Paper

Title

ANALYSIS OF HLA-G TRANSCRIPTS IN PBMCS OF NORMAL AND LUPUS INDIVIDUALS AND EFFECT OF IFN-γ ON THE EXPRESSION OF HLA-G

Author(s)

ANSARI MOHAMMAD | ZAVARAN HOSSEINI A. | YARI FATEMEH | Issue Writer Certificate

Keywords

GAMA INTERFERONQ3

HUMAN LEUKOCYTE ANTIGEN-GQ2

SYSTEMIC LUPUS ERYTHEMATOSUSQ2

Abstract

Objective: HLA-G is a nonclassical major histocompatibility complex antigen and is expressed as seven isoforms, including four membrane bound (HLA-G1 to –G4) and three soluble (HLA-G5 to–G7) forms. The pattern of selective expression of HLA-G transcripts in tissues shows the existence of a tight transcriptional control on the gene expression. It has been revealed that cytokines including interfrons and IL-10 could cause stimulation of the HLA-G transcription. The purpose of this study was to examine the effects of IFN-g on the expression of HLA-G transcripts in both PBMCs of normal and SLE patients. Materials and Methods: Whole blood of 20 female SLE patients and 15 healthy donor candidates for Bone Marrow Transplantation were used. PBMCs were isolated from the whole blood by Ficoll gradient centrifugation and cultured with or without IFN-g/LPS for 48 hours. Total RNA was extracted from the cells by trizol method. After reverse transcription of RNA to cDNA and the performance of a multiplex PCR for beta actin and HLA-G, the PCR products were analyzed using electrophoresis on a 2 % agarose gel and stained with ethidium bromide. Results: The results showed that the transcription of HLA-G was higher in SLE patients compared to normal controls. Addition of IFN-/LPS could influence the expression of this molecule by increasing the transcription of HLA-G in both normal and patient PBMCs (P£0.05). Conclusion: Transcription of HLA-G gene could be increased by the cytokine IFN-g. This observation is in accordance with previous reports. This effect could be assigned to both normal and lupus Patients. The effects of the cytokine IFN-g/LPS in the induction of HLA-G transcription were higher in normal than lupus patients. Nevertheless, the total expression of HLA-G was higher in lupus patients.

Cites

No record.

References

No record.

Cite

APA: Copy

ANSARI, MOHAMMAD, ZAVARAN HOSSEINI, A., & YARI, FATEMEH. (2010). ANALYSIS OF HLA-G TRANSCRIPTS IN PBMCS OF NORMAL AND LUPUS INDIVIDUALS AND EFFECT OF IFN-γ ON THE EXPRESSION OF HLA-G. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 12(4), 11-18. SID. https://sid.ir/paper/81240/en

Vancouver: Copy

ANSARI MOHAMMAD, ZAVARAN HOSSEINI A., YARI FATEMEH. ANALYSIS OF HLA-G TRANSCRIPTS IN PBMCS OF NORMAL AND LUPUS INDIVIDUALS AND EFFECT OF IFN-γ ON THE EXPRESSION OF HLA-G. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2010;12(4):11-18. Available from: https://sid.ir/paper/81240/en

IEEE: Copy

MOHAMMAD ANSARI, A. ZAVARAN HOSSEINI, and FATEMEH YARI, “ANALYSIS OF HLA-G TRANSCRIPTS IN PBMCS OF NORMAL AND LUPUS INDIVIDUALS AND EFFECT OF IFN-γ ON THE EXPRESSION OF HLA-G,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 12, no. 4, pp. 11–18, 2010, [Online]. Available: https://sid.ir/paper/81240/en

Related Journal Papers