EVALUATION OF INTRAMUSCULAR VERSUS INTRA-DERMAL INOCULATION OF BACTERIAL PLASMID ENCODING NUCLEOPROTEIN OF INFLUENZA VIRUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAMALI ABAS; SABAHI F.; BAMDAD T.; HASHEMI H.R.; MAHBOUDI FEREYDOUN; TAVASOTI KHEYRI M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2010 | Volume:13 | Issue:1 Page(s): 17-23

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

899

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF INTRAMUSCULAR VERSUS INTRA-DERMAL INOCULATION OF BACTERIAL PLASMID ENCODING NUCLEOPROTEIN OF INFLUENZA VIRUS

Author(s)

Keywords

CYTOTOXIC T LYMPHOCYTESQ3

GENETIC VACCINEQ3

INFLUENZA VIRUS NUCLEOPROTEINQ3

INTRA-DERMAL INOCULATIONQ3

INTRAMUSCULAR INOCULATIONQ2

Abstract

Objective: The use of bacterial plasmids carrying specific genes of pathogens as GENETIC VACCINEs is a relatively new technique for induction of cellular immune responses against microbial pathogens. Mechanisms of production of specific immune responses against these vaccines are not still completely understood. Therefore, it is necessary to examine various routes of inoculation to find the best way of immunization for specific antigens. In this research, intramuscular method of inoculation of influenza vaccine nucleoprotein (NP) encoding vector was compared with that of intra-dermal method. Materials and Methods: In this study, the ability of two different methods of immunization (intramuscular and intra-dermal) in induction of CTL responses as well as their efficiency in clearance of influenza virus from the lung of BALB/c mice was compared. Female BALB/c mice were immunized with influenza virus NP expressing plasmids on days 0, 14 and 28. CTL activity of mice was evaluated by lactate dehydrogenase technique two weeks after the last inoculation. In addition, the mice were challenged by live influenza virus and the viral titer was measured 4 days post-challenge in the lungs of animals. The results of experiments demonstrated that intramuscular immunization of mice induces a stronger CTL response. Mice immunized by intramuscular route also showed a higher ability in virus clearance from the lungs. Conclusion: Results of this study showed that different routes of immunization of influenza NP GENETIC VACCINE induce different levels of cell-mediated immune responses and protection from the live virus.

Cites

No record.

References

Cite

APA: Copy

JAMALI, ABAS, SABAHI, F., BAMDAD, T., HASHEMI, H.R., MAHBOUDI, FEREYDOUN, & TAVASOTI KHEYRI, M.. (2010). EVALUATION OF INTRAMUSCULAR VERSUS INTRA-DERMAL INOCULATION OF BACTERIAL PLASMID ENCODING NUCLEOPROTEIN OF INFLUENZA VIRUS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 13(1), 17-23. SID. https://sid.ir/paper/81251/en

Vancouver: Copy

JAMALI ABAS, SABAHI F., BAMDAD T., HASHEMI H.R., MAHBOUDI FEREYDOUN, TAVASOTI KHEYRI M.. EVALUATION OF INTRAMUSCULAR VERSUS INTRA-DERMAL INOCULATION OF BACTERIAL PLASMID ENCODING NUCLEOPROTEIN OF INFLUENZA VIRUS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2010;13(1):17-23. Available from: https://sid.ir/paper/81251/en

IEEE: Copy

ABAS JAMALI, F. SABAHI, T. BAMDAD, H.R. HASHEMI, FEREYDOUN MAHBOUDI, and M. TAVASOTI KHEYRI, “EVALUATION OF INTRAMUSCULAR VERSUS INTRA-DERMAL INOCULATION OF BACTERIAL PLASMID ENCODING NUCLEOPROTEIN OF INFLUENZA VIRUS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 13, no. 1, pp. 17–23, 2010, [Online]. Available: https://sid.ir/paper/81251/en

Related Journal Papers