COMPARATIVE STUDY OF CYTOTOXIC EFFECT OF ENTEROHEMORRHAGIC ESCHERICHIA COLI VEROTOXIN1 ON VERO AND RAJI CELL LINES

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DOKTORZADEH F.; SATARI MORTEZA; ZAHIR MOHAMMAD HASSAN; ZARIN GHALAM MOGHADAM M.

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Journal: Pathobiology Research Year:2010 | Volume:13 | Issue:1 Page(s): 47-55

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Title

COMPARATIVE STUDY OF CYTOTOXIC EFFECT OF ENTEROHEMORRHAGIC ESCHERICHIA COLI VEROTOXIN1 ON VERO AND RAJI CELL LINES

Author(s)

DOKTORZADEH F. | SATARI MORTEZA | ZAHIR MOHAMMAD HASSAN | ZARIN GHALAM MOGHADAM M. | Issue Writer Certificate

Keywords

ENTEROHEMORRHAGIC ESCHERICHIA COLIQ3

MTTQ3

RAJI CELL LINEQ3

VERO CELL LINEQ3

VEROTOXINQ2

Abstract

Objective: VEROTOXIN is a member of Shiga toxin family. This family contains AB protein toxins with an enzymatic (A) and a binding (B) compartment. Cells that have receptor (Gb3) are sensitive to cytotoxic effects of toxin. It has been shown that various tumor cells have Gb3 receptor and are selectively sensitive to apoptotic effect of VEROTOXIN. Studies on tumor cell lines and laboratory animals have shown antineoplastic and antiangiogenesis effects of this toxin. The aim of this study was comparison of cytotoxic effect of VEROTOXIN 1 on two cell lines: Vero (gold standard for evaluation of cytotoxic effect of VEROTOXIN) and Raji (a cell of a cultured line of lymphoblastoid cells derived from a Human Burkitt's lymphoma patients). Materials and Methods: The toxigenic strain was cultured and the production of toxin was evaluated by reverse passive latex agglutination test. VEROTOXIN 1 was purified by affinity chromatography. Vero and Raji cell were treated with serial dilutions of toxin, and viability was evaluated by MTT test. Results: Our result indicated that VEROTOXIN has cytotoxic effect on RAJI CELL LINEs. This effect is directly related to toxin concentration. Differences on cytotoxicity of toxin on Raji cells at 1:4-1:128 dilutions in relation to cytotoxicity of toxin on Vero cells at the same dilutions were considered statistically significant (P<0.05). But difference of cytotoxicity of toxin at higher dilutions was not significant. Conclusion: Our results revealed that VEROTOXIN has cytotoxicity on Vero and Raji cells, and this effect on Vero cells is more than Raji cells (P<0.05).

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APA: Copy

DOKTORZADEH, F., SATARI, MORTEZA, ZAHIR, MOHAMMAD HASSAN, & ZARIN GHALAM MOGHADAM, M.. (2010). COMPARATIVE STUDY OF CYTOTOXIC EFFECT OF ENTEROHEMORRHAGIC ESCHERICHIA COLI VEROTOXIN1 ON VERO AND RAJI CELL LINES. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 13(1), 47-55. SID. https://sid.ir/paper/81253/en

Vancouver: Copy

DOKTORZADEH F., SATARI MORTEZA, ZAHIR MOHAMMAD HASSAN, ZARIN GHALAM MOGHADAM M.. COMPARATIVE STUDY OF CYTOTOXIC EFFECT OF ENTEROHEMORRHAGIC ESCHERICHIA COLI VEROTOXIN1 ON VERO AND RAJI CELL LINES. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2010;13(1):47-55. Available from: https://sid.ir/paper/81253/en

IEEE: Copy

F. DOKTORZADEH, MORTEZA SATARI, MOHAMMAD HASSAN ZAHIR, and M. ZARIN GHALAM MOGHADAM, “COMPARATIVE STUDY OF CYTOTOXIC EFFECT OF ENTEROHEMORRHAGIC ESCHERICHIA COLI VEROTOXIN1 ON VERO AND RAJI CELL LINES,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 13, no. 1, pp. 47–55, 2010, [Online]. Available: https://sid.ir/paper/81253/en

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