EVALUATION OF RELATION BETWEEN MUTANT VIRB2 VIRULENCE FACTOR IN BRUCELLA MELITENSIS WITH IMMUNOGENICITY AND INTRACELLULAR SURVIVAL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

POURYASIN ALI; ROUZEHEI S.R.; SHOKRGOZAR M.A.; JAVADI GH.R.; SADEGHIZADEH MAJID

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2009 | Volume:12 | Issue:2 Page(s): 13-28

Download Full-Text

Persian Verion

View:

830

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF RELATION BETWEEN MUTANT VIRB2 VIRULENCE FACTOR IN BRUCELLA MELITENSIS WITH IMMUNOGENICITY AND INTRACELLULAR SURVIVAL

Author(s)

Keywords

BRUCELLA MELITENSISQ3

VIRB2 MUTATIONQ3

T4SSQ3

INTRACELLULAR REPLICATIONQ3

IMMUNOGENICITYQ3

Abstract

Objective: The BRUCELLA MELITENSIS virB operon, encoding a type IV secretion system (T4SS), is required for INTRACELLULAR REPLICATION and persistent infection in the mouse model. The product of the second gene of the virB operon, virB2, is predicted to be localized at the bacterial surface, where they could potentially interact with host cells. Studies to date have focused on characterization of transposon mutations in this gene, which is expected to exert polar effects on downstream genes. We researched on the evaluation of relation between virB2 mutant with IMMUNOGENICITY in mouse model and INTRACELLULAR REPLICATION in macrophages J774.Materials and Methods: In order to determine whether VirB2 is required for the function of the T4SS apparatus, we constructed and characterized deletion mutation of virB2 and kanamycin resistance gene replaced instead of virB2. For demonstration of INTRACELLULAR REPLICATION, macrophages J774 and BALB/c mices were infected with wild type BRUCELLA MELITENSIS and mutated. Results: After 48 h, number of mutated Brucella severe decreased severly compaired to wild type in macrophages J774, and Brucella with virB2 deletion decreased from 1×106 CFU/spleen less than 1000 CFU/spleen during 8 weeks, also total IgG increased in both but IL-12 and IFN-g increased only in wild type. Conclusion: VirB2 was essential for INTRACELLULAR REPLICATION in mouse models and J774 macrophages. The virB2 mutant was unable to cause persistent infection in the mouse model, demonstrating the essential role of VirB2 in the function of the T4SS apparatus during infection and secreting IL-12 and IFN-g, but VirB2 dispensable for secretion of total IgG.

Cites

No record.

References

No record.

Cite

APA: Copy

POURYASIN, ALI, ROUZEHEI, S.R., SHOKRGOZAR, M.A., JAVADI, GH.R., & SADEGHIZADEH, MAJID. (2009). EVALUATION OF RELATION BETWEEN MUTANT VIRB2 VIRULENCE FACTOR IN BRUCELLA MELITENSIS WITH IMMUNOGENICITY AND INTRACELLULAR SURVIVAL. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 12(2), 13-28. SID. https://sid.ir/paper/81310/en

Vancouver: Copy

POURYASIN ALI, ROUZEHEI S.R., SHOKRGOZAR M.A., JAVADI GH.R., SADEGHIZADEH MAJID. EVALUATION OF RELATION BETWEEN MUTANT VIRB2 VIRULENCE FACTOR IN BRUCELLA MELITENSIS WITH IMMUNOGENICITY AND INTRACELLULAR SURVIVAL. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2009;12(2):13-28. Available from: https://sid.ir/paper/81310/en

IEEE: Copy

ALI POURYASIN, S.R. ROUZEHEI, M.A. SHOKRGOZAR, GH.R. JAVADI, and MAJID SADEGHIZADEH, “EVALUATION OF RELATION BETWEEN MUTANT VIRB2 VIRULENCE FACTOR IN BRUCELLA MELITENSIS WITH IMMUNOGENICITY AND INTRACELLULAR SURVIVAL,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 12, no. 2, pp. 13–28, 2009, [Online]. Available: https://sid.ir/paper/81310/en

Related Journal Papers